3SAM
Structure of D13, the scaffolding protein of vaccinia virus (mutant D513G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.953689 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 189.630, 189.630, 255.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.990 - 2.550 |
| R-factor | 0.1763 |
| Rwork | 0.175 |
| R-free | 0.20080 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.079 | 0.102 |
| Number of reflections | 87870 | |
| <I/σ(I)> | 26.25 | 2.25 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 10.9 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.3M Na formate, 100mM Bis-Tris pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
