3S4T

Crystal structure of putative amidohydrolase-2 (EFI-target 500288)from Polaromonas sp. JS666

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Amidohydrolase 2	polymer	348	40631.3	8	UniProt (Q12BV1) Pfam (PF04909)	Polaromonas sp. JS666
2	DA, I, Q, QA, RA... (D, A, B, F, G)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	7	Chemie (EPE)
3	AB, BB, CB, DB, EA... (G, D, H, A, E...)	SULFATE ION	non-polymer		96.1	30	Chemie (SO4)
4	AA, BA, EB, FB, GB... (C, G, D, H, A...)	ACETATE ION	non-polymer		59.0	16	Chemie (ACT)
5	X (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	CA, JA, VA (C, D, F)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
7	NB, OB, PB, QB, RB... (A, B, C, D, E...)	water	water		18.0	1513	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 326 (UniProt: Q12BV1)

PDB	External Database	Details
Ala 327	-	expression tag
Glu 328	-	expression tag
Asn 329	-	expression tag
Leu 330	-	expression tag
Tyr 331	-	expression tag
Phe 332	-	expression tag
Gln 333	-	expression tag
Ser 334	-	expression tag
His 335	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
Trp 341	-	expression tag
Ser 342	-	expression tag
His 343	-	expression tag
Pro 344	-	expression tag
Gln 345	-	expression tag
Phe 346	-	expression tag
Glu 347	-	expression tag
Lys 348	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	325050.6
Non-Polymers*	Number of molecules	57
	Total formula weight	5806.5
All*	Total formula weight	330857.1

*Water molecules are not included.