Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3S4T

Crystal structure of putative amidohydrolase-2 (EFI-target 500288)from Polaromonas sp. JS666

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0016787	molecular_function	hydrolase activity
A	0016831	molecular_function	carboxy-lyase activity
A	0019748	biological_process	secondary metabolic process
A	0046872	molecular_function	metal ion binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0016787	molecular_function	hydrolase activity
B	0016831	molecular_function	carboxy-lyase activity
B	0019748	biological_process	secondary metabolic process
B	0046872	molecular_function	metal ion binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0016787	molecular_function	hydrolase activity
C	0016831	molecular_function	carboxy-lyase activity
C	0019748	biological_process	secondary metabolic process
C	0046872	molecular_function	metal ion binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0016787	molecular_function	hydrolase activity
D	0016831	molecular_function	carboxy-lyase activity
D	0019748	biological_process	secondary metabolic process
D	0046872	molecular_function	metal ion binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0016787	molecular_function	hydrolase activity
E	0016831	molecular_function	carboxy-lyase activity
E	0019748	biological_process	secondary metabolic process
E	0046872	molecular_function	metal ion binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0016787	molecular_function	hydrolase activity
F	0016831	molecular_function	carboxy-lyase activity
F	0019748	biological_process	secondary metabolic process
F	0046872	molecular_function	metal ion binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0016787	molecular_function	hydrolase activity
G	0016831	molecular_function	carboxy-lyase activity
G	0019748	biological_process	secondary metabolic process
G	0046872	molecular_function	metal ion binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0016787	molecular_function	hydrolase activity
H	0016831	molecular_function	carboxy-lyase activity
H	0019748	biological_process	secondary metabolic process
H	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EPE A 400

Chain	Residue
A	PRO25
A	ASP26
A	LYS27
B	THR239
B	THR240
B	LYS242

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EPE B 400

Chain	Residue
B	ASP26
B	LYS27
A	THR239
A	THR240
B	PRO25

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EPE D 400

Chain	Residue
C	PRO25
C	ASP26
C	LYS27
D	THR239
D	THR240
D	LYS242

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EPE F 400

Chain	Residue
E	PRO25
E	ASP26
E	LYS27
F	THR239
F	THR240
F	LYS242
F	HOH1273

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EPE F 349

Chain	Residue
E	THR239
E	THR240
E	LYS242
F	PRO25
F	ASP26
F	LYS27
F	HOH1449

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EPE G 400

Chain	Residue
G	PRO25
G	ASP26
G	LYS27
G	HOH1097
H	THR239
H	THR240
H	LYS242

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EPE G 349

Chain	Residue
G	THR239
G	THR240
G	LYS242
H	PRO25
H	ASP26
H	LYS27

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 F 350

Chain	Residue
F	ARG42
F	ARG45
F	ACT351
F	HOH1188

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 349

Chain	Residue
B	ARG42
B	ARG45
B	HOH1568

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 349

Chain	Residue
D	ARG42
D	ARG45
D	ACT353
D	HOH900

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 H 349

Chain	Residue
H	ARG42
H	ARG45
H	HOH933

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 349

Chain	Residue
A	PHE23
A	ARG166
A	ASN167

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 E 349

Chain	Residue
E	ARG174
E	HOH483
E	HOH641
E	HOH1456
F	SER171

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 350

Chain	Residue
C	ARG174
D	LEU169
D	SER171
D	HOH362

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 E 350

Chain	Residue
E	ARG42
E	ARG45
E	ACT353
E	HOH1079

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 G 350

Chain	Residue
G	PHE23
G	PRO62
G	ARG166
G	ASN167

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 G 351

Chain	Residue
G	ARG42
G	ARG45
G	ACT356
G	HOH1317

site_id	BC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 H 350

Chain	Residue
G	ARG174
H	LEU169
H	SER171
H	HOH546

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 350

Chain	Residue
A	ARG42
A	ARG45
A	LEU46
A	HOH1594

site_id	CC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 350

Chain	Residue
A	ARG174
B	LEU169
B	SER171
B	HOH646

site_id	CC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 E 351

Chain	Residue
E	GLN132
E	LEU169
E	HOH1087
E	HOH1241
E	HOH1523

site_id	CC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 351

Chain	Residue
D	PRO30
D	ARG33

site_id	CC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 352

Chain	Residue
D	ARG42
D	ARG45
D	HOH1440

site_id	CC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 349

Chain	Residue
C	HIS232
C	LYS248

site_id	CC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 H 351

Chain	Residue
H	ARG78
H	ARG115

site_id	CC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 G 352

Chain	Residue
G	ARG247
G	ASP251
G	HOH1110

site_id	CC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 351

Chain	Residue
B	PRO30
B	ARG33

site_id	DC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 E 352

Chain	Residue
E	PHE23
E	PRO62
E	ARG166
E	ASN167
E	HOH1333

site_id	DC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 351

Chain	Residue
A	ARG247

site_id	DC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 352

Chain	Residue
B	ARG78
B	ARG115

site_id	DC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 H 352

Chain	Residue
H	ARG42
H	ARG45

site_id	DC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 350

Chain	Residue
C	PHE23
C	PRO62
C	ARG166
C	ASN167
C	HOH376

site_id	DC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 H 353

Chain	Residue
H	PHE23
H	PRO62
H	ARG166
H	ASN167
H	PHE189
H	HOH718

site_id	DC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 G 353

Chain	Residue
G	GLN132
G	HOH451
G	HOH1166

site_id	DC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 G 354

Chain	Residue
G	ARG78
G	ARG115

site_id	DC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 352

Chain	Residue
A	HOH1065
C	ARG42
C	ARG45

site_id	EC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 G 355

Chain	Residue
D	ARG78
G	LYS323
G	ARG326

site_id	EC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT D 353

Chain	Residue
D	TRP288
D	ASN292
D	ILE293
D	ASP294
D	SO4349
D	HOH900

site_id	EC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACT G 356

Chain	Residue
G	LEU46
G	TRP288
G	ASN292
G	ILE293
G	ASP294
G	SO4351
G	HOH1317

site_id	EC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT E 353

Chain	Residue
E	TRP288
E	ASN292
E	ILE293
E	ASP294
E	SO4350
E	HOH1079

site_id	EC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ACT H 354

Chain	Residue
H	TRP288
H	ASN292
H	ILE293
H	ASP294
H	HOH933

site_id	EC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT A 353

Chain	Residue
A	LEU46
A	TRP288
A	ASN292
A	ILE293
A	ASP294
A	HOH1594

site_id	EC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT C 351

Chain	Residue
A	HOH1065
C	LEU46
C	TRP288
C	ASN292
C	ILE293
C	ASP294

site_id	EC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ACT B 353

Chain	Residue
B	TRP288
B	ASN292
B	ILE293
B	ASP294
B	HOH1568

site_id	EC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT C 352

Chain	Residue
C	HIS295
C	HOH868
D	LYS238
D	HOH1043

site_id	FC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT F 351

Chain	Residue
F	TRP288
F	ASN292
F	ILE293
F	ASP294
F	SO4350
F	HOH1188

site_id	FC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE ACT B 354

Chain	Residue
B	ARG45

site_id	FC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE ACT F 352

Chain	Residue
F	ARG45

site_id	FC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT A 354

Chain	Residue
A	HIS295
A	HOH743
B	LYS238
B	HOH1042

site_id	FC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT G 357

Chain	Residue
G	HIS295
G	HOH1044
H	LYS238

site_id	FC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE ACT G 358

Chain	Residue
G	HIS232

site_id	FC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT A 355

Chain	Residue
A	LYS238
A	HOH369
B	HIS295

site_id	FC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT E 354

Chain	Residue
E	HIS295
E	HOH870
E	HOH1139
F	LYS238

site_id	FC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL F 353

Chain	Residue
F	PHE23
F	PRO62
F	ARG166
F	ASN167

site_id	GC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL C 353

Chain	Residue
C	GLY178
C	HIS179
C	ALA180
C	HOH401

site_id	GC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL D 354

Chain	Residue
D	PHE23
D	ARG166
D	ASN167
D	HOH619

site_id	GC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL B 355

Chain	Residue
B	ASN167
B	HOH1406

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: ACT_SITE => ECO:0000303\|PubMed:29283551

Chain	Residue	Details
A	ASP287
B	ASP287
C	ASP287
D	ASP287
E	ASP287
F	ASP287
G	ASP287
H	ASP287

site_id	SWS_FT_FI2
Number of Residues	32
Details	BINDING: BINDING => ECO:0000269\|PubMed:29283551, ECO:0007744\|PDB:4QRO

Chain	Residue	Details
A	GLU8
C	HIS10
C	HIS164
C	ASP287
D	GLU8
D	HIS10
D	HIS164
D	ASP287
E	GLU8
E	HIS10
E	HIS164
A	HIS10
E	ASP287
F	GLU8
F	HIS10
F	HIS164
F	ASP287
G	GLU8
G	HIS10
G	HIS164
G	ASP287
H	GLU8
A	HIS164
H	HIS10
H	HIS164
H	ASP287
A	ASP287
B	GLU8
B	HIS10
B	HIS164
B	ASP287
C	GLU8

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)