3S44

Crystal Structure of Pasteurella multocida sialyltransferase M144D mutant with CMP bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alpha-2,3/2,6-sialyltransferase/sialidase	polymer	399	46451.4	1	UniProt (Q15KI8) Pfam (PF11477) In PDB	Pasteurella multocida
2	A	CMP-3F(a)-Neu5Ac	non-polymer		588.4	1	Chemie (FN5)
3		water	water		18.0	431	Chemie (HOH)

Sequence modifications

A: 26 - 412 (UniProt: Q15KI8)

PDB	External Database	Details
Met 25	-	initiating methionine
Asp 144	Met 144	engineered mutation
Gly 413	-	expression tag
Gly 414	-	expression tag
Gly 415	-	expression tag
Leu 416	-	expression tag
Glu 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46451.4
Non-Polymers*	Number of molecules	1
	Total formula weight	588.4
All*	Total formula weight	47039.8

*Water molecules are not included.