3S3O
Crystal structure of the Prototype Foamy Virus (PFV) N224H mutant intasome in complex with magnesium and Dolutegravir (S/GSK1349572)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | PFV integrase | polymer | 395 | 44480.7 | 2 | UniProt (P14350) Pfam (PF17921) Pfam (PF00665) Pfam (PF18103) | Human spumaretrovirus (SFVcpz(hu) Human foamy virus) | IN, p42In |
| 2 | C (C) | 5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP*TP*CP*GP*CP*A)-3' | polymer | 19 | 5834.8 | 1 | |||
| 3 | D (D) | 5'-D(*TP*GP*CP*GP*AP*AP*AP*TP*TP*CP*CP*AP*TP*GP*AP*CP*A)-3' | polymer | 17 | 5195.4 | 1 | |||
| 4 | E (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 5 | F, N, P (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 6 | G, H, I, J (A) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 7 | K (A) | AMMONIUM ION | non-polymer | 18.0 | 1 | Chemie (NH4) | |||
| 8 | L, M (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 9 | O (A) | (4R,12aS)-N-(2,4-difluorobenzyl)-7-hydroxy-4-methyl-6,8-dioxo-3,4,6,8,12,12a-hexahydro-2H-pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazine-9-carboxamide | non-polymer | 419.4 | 1 | Chemie (DLU) | |||
| 10 | Q (B) | HEXANE-1,6-DIOL | non-polymer | 118.2 | 1 | Chemie (HEZ) | |||
| 11 | R, S, T, U (A, B, C, D) | water | water | 18.0 | 199 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 392 (UniProt: P14350)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Pro -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ser 217 | Gly 968 | engineered mutation |
| Gly 218 | Ser 969 | variant |
| His 224 | Asn 975 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 99991.7 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1326.2 | |
| All* | Total formula weight | 101317.8 |
