3S19
Crystal Structure of the R262L mutant of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with preQ0
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | NADPH-dependent 7-cyano-7-deazaguanine reductase | polymer | 290 | 33271.3 | 4 | UniProt (Q9KTK0) Pfam (PF14819) Pfam (PF14489) In PDB | Vibrio cholerae | 7-cyano-7-carbaguanine reductase, NADPH-dependent nitrile oxidoreductase, PreQ(0) reductase |
| 2 | A, B, C, D | 7-DEAZA-7-AMINOMETHYL-GUANINE | non-polymer | 179.2 | 4 | Chemie (PRF) | |||
| 3 | A, B, C, D | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 4 | water | water | 18.0 | 1192 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 7 - 287 (UniProt: Q9KTK0)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Mse 1 | - | expression tag |
| Asn 2 | - | expression tag |
| Arg 3 | - | expression tag |
| Leu 4 | - | expression tag |
| Lys 5 | - | expression tag |
| Asn 6 | - | expression tag |
| Leu 262 | Arg 256 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 133085.1 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1085.1 | |
| All* | Total formula weight | 134170.2 |
