3S19
Crystal Structure of the R262L mutant of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with preQ0
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003674 | molecular_function | molecular_function |
| A | 0005575 | cellular_component | cellular_component |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006400 | biological_process | tRNA modification |
| A | 0008150 | biological_process | biological_process |
| A | 0008616 | biological_process | queuosine biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0033739 | molecular_function | preQ1 synthase activity |
| A | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| B | 0003674 | molecular_function | molecular_function |
| B | 0005575 | cellular_component | cellular_component |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006400 | biological_process | tRNA modification |
| B | 0008150 | biological_process | biological_process |
| B | 0008616 | biological_process | queuosine biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0033739 | molecular_function | preQ1 synthase activity |
| B | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| C | 0003674 | molecular_function | molecular_function |
| C | 0005575 | cellular_component | cellular_component |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006400 | biological_process | tRNA modification |
| C | 0008150 | biological_process | biological_process |
| C | 0008616 | biological_process | queuosine biosynthetic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0033739 | molecular_function | preQ1 synthase activity |
| C | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| D | 0003674 | molecular_function | molecular_function |
| D | 0005575 | cellular_component | cellular_component |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006400 | biological_process | tRNA modification |
| D | 0008150 | biological_process | biological_process |
| D | 0008616 | biological_process | queuosine biosynthetic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0033739 | molecular_function | preQ1 synthase activity |
| D | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE PRF A 290 |
| Chain | Residue |
| A | TRP62 |
| A | GLU234 |
| A | ILE267 |
| A | LEU92 |
| A | ILE93 |
| A | GLU94 |
| A | SER95 |
| A | CYS194 |
| A | ASP201 |
| A | PHE232 |
| A | HIS233 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 291 |
| Chain | Residue |
| A | ALA90 |
| A | ASN91 |
| A | HOH665 |
| D | ASN91 |
| D | HOH690 |
| D | HOH836 |
| site_id | AC3 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE PRF B 290 |
| Chain | Residue |
| B | TRP62 |
| B | LEU92 |
| B | ILE93 |
| B | GLU94 |
| B | SER95 |
| B | CYS194 |
| B | ILE196 |
| B | ASP201 |
| B | PHE232 |
| B | HIS233 |
| B | GLU234 |
| B | LEU262 |
| B | ILE267 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL B 291 |
| Chain | Residue |
| B | THR63 |
| B | TYR65 |
| B | GLU82 |
| B | TYR142 |
| B | ARG258 |
| B | ASN281 |
| B | ARG283 |
| site_id | AC5 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE PRF C 290 |
| Chain | Residue |
| C | TRP62 |
| C | LEU92 |
| C | ILE93 |
| C | GLU94 |
| C | SER95 |
| C | CYS194 |
| C | ILE196 |
| C | ASP201 |
| C | GLU231 |
| C | PHE232 |
| C | HIS233 |
| C | GLU234 |
| C | LEU262 |
| C | ILE267 |
| site_id | AC6 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL C 291 |
| Chain | Residue |
| C | THR63 |
| C | TYR65 |
| C | GLU82 |
| C | TYR142 |
| C | ARG258 |
| C | ASN281 |
| C | ARG283 |
| C | HOH518 |
| C | HOH897 |
| site_id | AC7 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE PRF D 290 |
| Chain | Residue |
| D | TRP62 |
| D | LEU92 |
| D | ILE93 |
| D | GLU94 |
| D | SER95 |
| D | CYS194 |
| D | ILE196 |
| D | ASP201 |
| D | PHE232 |
| D | HIS233 |
| D | GLU234 |
| D | ILE267 |
| site_id | AC8 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL D 291 |
| Chain | Residue |
| D | THR63 |
| D | TYR65 |
| D | GLU82 |
| D | TYR142 |
| D | ARG258 |
| D | ASN281 |
| D | ARG283 |
| D | HOH789 |
| D | HOH1171 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Thioimide intermediate => ECO:0000255|HAMAP-Rule:MF_00817, ECO:0000269|Ref.3 |
| Chain | Residue | Details |
| A | CYS194 | |
| B | CYS194 | |
| C | CYS194 | |
| D | CYS194 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_00817 |
| Chain | Residue | Details |
| A | ASP201 | |
| B | ASP201 | |
| C | ASP201 | |
| D | ASP201 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | ILE93 | |
| C | SER95 | |
| C | HIS233 | |
| C | LEU262 | |
| D | ILE93 | |
| D | SER95 | |
| D | HIS233 | |
| D | LEU262 | |
| A | SER95 | |
| A | HIS233 | |
| A | LEU262 | |
| B | ILE93 | |
| B | SER95 | |
| B | HIS233 | |
| B | LEU262 | |
| C | ILE93 |
