3RWT

Crystal structure of circular permutated Red Fluorescent Protein mKate(cp 154-153)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (F, A, B, C, D...)	Fluorescent protein FP480,Fluorescent protein FP480	polymer	237	26586.3	8	UniProt (D0VX33) Pfam (PF01353)	Entacmaea quadricolor (Bubble-tip anemone)
2	I (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	J, K (F, A)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

F, A, B, C, D, E, G, H: 0 - 79 (UniProt: D0VX33)

PDB	External Database	Details
Glu 0	Gly 152	engineered mutation
Phe 1	Gly 153	engineered mutation
Ser 6	Ala 158	conflict
Met 8	Leu 160	conflict
Leu 22	Phe 174	conflict
Arg 45	Tyr 197	conflict

F, A, B, C, D, E, G, H: 86 - 236 (UniProt: D0VX33)

PDB	External Database	Details
Gly 80	-	linker
Gly 81	-	linker
Thr 82	-	linker
Gly 83	-	linker
Gly 84	-	linker
Ser 85	-	linker
Met 126	Gln 41	conflict
-	Met 63	chromophore
-	Tyr 64	chromophore
Nrq 148	Gly 65	chromophore
Lys 152	His 67	conflict
Phe 165	Trp 80	conflict
Ser 228	His 143	conflict

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	212690.1
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	212714.4

*Water molecules are not included.