3RWT
Crystal structure of circular permutated Red Fluorescent Protein mKate(cp 154-153)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2009-11-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.457, 71.441, 367.727 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 - 3.000 |
| R-factor | 0.2203 |
| Rwork | 0.217 |
| R-free | 0.27840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.590 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 3.103 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.440 | |
| Number of reflections | 38875 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 89.0 | 100 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1 M Tris-HCl pH 7.4, 0.2 M MgCl2 and 18% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
