3RO0

Crystal structure of Bacillus amyloliquefaciens pyroglutamyl peptidase I and terpyridine platinum(II)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Pyrrolidone-carboxylate peptidase	polymer	223	24376.7	4	UniProt (P46107) Pfam (PF01470)	Bacillus amyloliquefaciens	5-oxoprolyl-peptidase, Pyroglutamyl-peptidase I, PGP-I, Pyrase
2	E, F, G, H, I (A, B, C, D)	2,2':6',2''-TERPYRIDINE PLATINUM(II) Chloride	non-polymer		463.8	5	Chemie (TPT)
3	J, K, L, M (A, B, C, D)	water	water		18.0	958	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 215 (UniProt: P46107)

PDB	External Database	Details
Met 58	Ile 58	engineered mutation
Ala 202	Val 202	engineered mutation
Leu 216	-	expression tag
Glu 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	97506.8
Non-Polymers*	Number of molecules	5
	Total formula weight	2319.0
All*	Total formula weight	99825.8

*Water molecules are not included.