3RNM
The crystal structure of the subunit binding of human dihydrolipoamide transacylase (E2b) bound to human dihydrolipoamide dehydrogenase (E3)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Dihydrolipoyl dehydrogenase, mitochondrial | polymer | 495 | 52652.1 | 4 | UniProt (P09622) Pfam (PF07992) Pfam (PF02852) | Homo sapiens (human) | Dihydrolipoamide dehydrogenase, Glycine cleavage system L protein |
| 2 | E, F (E, F) | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial | polymer | 58 | 6592.5 | 2 | UniProt (P11182) Pfam (PF02817) | Homo sapiens (human) | Branched-chain alpha-keto acid dehydrogenase complex component E2, BCKAD-E2, BCKADE2, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, Dihydrolipoamide branched chain transacylase, Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase |
| 3 | G, J, M, P (A, B, C, D) | BETA-MERCAPTOETHANOL | non-polymer | 78.1 | 4 | Chemie (BME) | |||
| 4 | H, K, N, Q (A, B, C, D) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 4 | Chemie (FAD) | |||
| 5 | I, L, O, R (A, B, C, D) | 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID | non-polymer | 207.3 | 4 | Chemie (NHE) | |||
| 6 | S, T, U, V, W... (A, B, C, D, E...) | water | water | 18.0 | 347 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 474 (UniProt: P09622)
E, F: 104 - 152 (UniProt: P11182)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | expression tag |
| Gly -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Met -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Ser -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Asn -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Tyr -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Gln 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 103 | - | expression tag |
| Asn 118 | Arg 179 | engineered mutation |
| Leu 153 | - | expression tag |
| Glu 154 | - | expression tag |
| His 155 | - | expression tag |
| His 156 | - | expression tag |
| His 157 | - | expression tag |
| His 158 | - | expression tag |
| His 159 | - | expression tag |
| His 160 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 223793.6 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 4283.9 | |
| All* | Total formula weight | 228077.5 |
