3RNM

The crystal structure of the subunit binding of human dihydrolipoamide transacylase (E2b) bound to human dihydrolipoamide dehydrogenase (E3)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Dihydrolipoyl dehydrogenase, mitochondrial	polymer	495	52652.1	4	UniProt (P09622) Pfam (PF07992) Pfam (PF02852) In PDB	Homo sapiens (human)	Dihydrolipoamide dehydrogenase, Glycine cleavage system L protein
2	E, F	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial	polymer	58	6592.5	2	UniProt (P11182) Pfam (PF02817) In PDB	Homo sapiens (human)	Branched-chain alpha-keto acid dehydrogenase complex component E2, BCKAD-E2, BCKADE2, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, Dihydrolipoamide branched chain transacylase, Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase
3	A, B, C, D	BETA-MERCAPTOETHANOL	non-polymer		78.1	4	Chemie (BME)
4	A, B, C, D	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	4	Chemie (FAD)
5	A, B, C, D	2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID	non-polymer		207.3	4	Chemie (NHE)
6		water	water		18.0	347	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 474 (UniProt: P09622)

PDB	External Database	Details
Met -20	-	EXPRESSION TAG
Gly -19	-	EXPRESSION TAG
Gly -18	-	EXPRESSION TAG
Ser -17	-	EXPRESSION TAG
His -16	-	EXPRESSION TAG
His -15	-	EXPRESSION TAG
His -14	-	EXPRESSION TAG
His -13	-	EXPRESSION TAG
His -12	-	EXPRESSION TAG
His -11	-	EXPRESSION TAG
Gly -10	-	EXPRESSION TAG
Met -9	-	EXPRESSION TAG
Ala -8	-	EXPRESSION TAG
Ser -7	-	EXPRESSION TAG
Leu -6	-	EXPRESSION TAG
Glu -5	-	EXPRESSION TAG
Asn -4	-	EXPRESSION TAG
Leu -3	-	EXPRESSION TAG
Tyr -2	-	EXPRESSION TAG
Phe -1	-	EXPRESSION TAG
Gln 0	-	EXPRESSION TAG

E, F: 104 - 152 (UniProt: P11182)

PDB	External Database	Details
Gly 103	-	EXPRESSION TAG
Asn 118	Arg 179	ENGINEERED MUTATION
Leu 153	-	EXPRESSION TAG
Glu 154	-	EXPRESSION TAG
His 155	-	EXPRESSION TAG
His 156	-	EXPRESSION TAG
His 157	-	EXPRESSION TAG
His 158	-	EXPRESSION TAG
His 159	-	EXPRESSION TAG
His 160	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	223793.6
Non-Polymers*	Number of molecules	12
	Total formula weight	4283.9
All*	Total formula weight	228077.5

*Water molecules are not included.