3RNM
The crystal structure of the subunit binding of human dihydrolipoamide transacylase (E2b) bound to human dihydrolipoamide dehydrogenase (E3)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001669 | cellular_component | acrosomal vesicle |
| A | 0004148 | molecular_function | dihydrolipoyl dehydrogenase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0005929 | cellular_component | cilium |
| A | 0005967 | cellular_component | obsolete mitochondrial pyruvate dehydrogenase complex |
| A | 0006086 | biological_process | acetyl-CoA biosynthetic process from pyruvate |
| A | 0006120 | biological_process | mitochondrial electron transport, NADH to ubiquinone |
| A | 0006508 | biological_process | proteolysis |
| A | 0007369 | biological_process | gastrulation |
| A | 0009083 | biological_process | branched-chain amino acid catabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016668 | molecular_function | oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor |
| A | 0031410 | cellular_component | cytoplasmic vesicle |
| A | 0031514 | cellular_component | motile cilium |
| A | 0034604 | molecular_function | pyruvate dehydrogenase (NAD+) activity |
| A | 0042391 | biological_process | regulation of membrane potential |
| A | 0043159 | cellular_component | acrosomal matrix |
| A | 0045240 | cellular_component | alpha-ketoacid dehydrogenase complex |
| A | 0045252 | cellular_component | oxoglutarate dehydrogenase complex |
| A | 0045254 | cellular_component | pyruvate dehydrogenase complex |
| A | 0047101 | molecular_function | branched-chain alpha-keto acid dehydrogenase activity |
| A | 0048240 | biological_process | sperm capacitation |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| A | 1902493 | cellular_component | acetyltransferase complex |
| B | 0001669 | cellular_component | acrosomal vesicle |
| B | 0004148 | molecular_function | dihydrolipoyl dehydrogenase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0005929 | cellular_component | cilium |
| B | 0005967 | cellular_component | obsolete mitochondrial pyruvate dehydrogenase complex |
| B | 0006086 | biological_process | acetyl-CoA biosynthetic process from pyruvate |
| B | 0006120 | biological_process | mitochondrial electron transport, NADH to ubiquinone |
| B | 0006508 | biological_process | proteolysis |
| B | 0007369 | biological_process | gastrulation |
| B | 0009083 | biological_process | branched-chain amino acid catabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016668 | molecular_function | oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor |
| B | 0031410 | cellular_component | cytoplasmic vesicle |
| B | 0031514 | cellular_component | motile cilium |
| B | 0034604 | molecular_function | pyruvate dehydrogenase (NAD+) activity |
| B | 0042391 | biological_process | regulation of membrane potential |
| B | 0043159 | cellular_component | acrosomal matrix |
| B | 0045240 | cellular_component | alpha-ketoacid dehydrogenase complex |
| B | 0045252 | cellular_component | oxoglutarate dehydrogenase complex |
| B | 0045254 | cellular_component | pyruvate dehydrogenase complex |
| B | 0047101 | molecular_function | branched-chain alpha-keto acid dehydrogenase activity |
| B | 0048240 | biological_process | sperm capacitation |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 1902493 | cellular_component | acetyltransferase complex |
| C | 0001669 | cellular_component | acrosomal vesicle |
| C | 0004148 | molecular_function | dihydrolipoyl dehydrogenase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005739 | cellular_component | mitochondrion |
| C | 0005759 | cellular_component | mitochondrial matrix |
| C | 0005929 | cellular_component | cilium |
| C | 0005967 | cellular_component | obsolete mitochondrial pyruvate dehydrogenase complex |
| C | 0006086 | biological_process | acetyl-CoA biosynthetic process from pyruvate |
| C | 0006120 | biological_process | mitochondrial electron transport, NADH to ubiquinone |
| C | 0006508 | biological_process | proteolysis |
| C | 0007369 | biological_process | gastrulation |
| C | 0009083 | biological_process | branched-chain amino acid catabolic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016668 | molecular_function | oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor |
| C | 0031410 | cellular_component | cytoplasmic vesicle |
| C | 0031514 | cellular_component | motile cilium |
| C | 0034604 | molecular_function | pyruvate dehydrogenase (NAD+) activity |
| C | 0042391 | biological_process | regulation of membrane potential |
| C | 0043159 | cellular_component | acrosomal matrix |
| C | 0045240 | cellular_component | alpha-ketoacid dehydrogenase complex |
| C | 0045252 | cellular_component | oxoglutarate dehydrogenase complex |
| C | 0045254 | cellular_component | pyruvate dehydrogenase complex |
| C | 0047101 | molecular_function | branched-chain alpha-keto acid dehydrogenase activity |
| C | 0048240 | biological_process | sperm capacitation |
| C | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| C | 1902493 | cellular_component | acetyltransferase complex |
| D | 0001669 | cellular_component | acrosomal vesicle |
| D | 0004148 | molecular_function | dihydrolipoyl dehydrogenase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005634 | cellular_component | nucleus |
| D | 0005739 | cellular_component | mitochondrion |
| D | 0005759 | cellular_component | mitochondrial matrix |
| D | 0005929 | cellular_component | cilium |
| D | 0005967 | cellular_component | obsolete mitochondrial pyruvate dehydrogenase complex |
| D | 0006086 | biological_process | acetyl-CoA biosynthetic process from pyruvate |
| D | 0006120 | biological_process | mitochondrial electron transport, NADH to ubiquinone |
| D | 0006508 | biological_process | proteolysis |
| D | 0007369 | biological_process | gastrulation |
| D | 0009083 | biological_process | branched-chain amino acid catabolic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016668 | molecular_function | oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor |
| D | 0031410 | cellular_component | cytoplasmic vesicle |
| D | 0031514 | cellular_component | motile cilium |
| D | 0034604 | molecular_function | pyruvate dehydrogenase (NAD+) activity |
| D | 0042391 | biological_process | regulation of membrane potential |
| D | 0043159 | cellular_component | acrosomal matrix |
| D | 0045240 | cellular_component | alpha-ketoacid dehydrogenase complex |
| D | 0045252 | cellular_component | oxoglutarate dehydrogenase complex |
| D | 0045254 | cellular_component | pyruvate dehydrogenase complex |
| D | 0047101 | molecular_function | branched-chain alpha-keto acid dehydrogenase activity |
| D | 0048240 | biological_process | sperm capacitation |
| D | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| D | 1902493 | cellular_component | acetyltransferase complex |
| E | 0016746 | molecular_function | acyltransferase activity |
| F | 0016746 | molecular_function | acyltransferase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE BME A 500 |
| Chain | Residue |
| A | SER168 |
| A | ILE189 |
| A | CYS277 |
| A | ARG280 |
| A | FAD480 |
| A | HOH563 |
| site_id | AC2 |
| Number of Residues | 39 |
| Details | BINDING SITE FOR RESIDUE FAD A 480 |
| Chain | Residue |
| A | PRO16 |
| A | GLY17 |
| A | GLU36 |
| A | LYS37 |
| A | ASN38 |
| A | GLY43 |
| A | THR44 |
| A | CYS45 |
| A | VAL48 |
| A | GLY49 |
| A | CYS50 |
| A | LYS54 |
| A | GLY117 |
| A | TYR118 |
| A | GLY119 |
| A | ALA147 |
| A | THR148 |
| A | GLY149 |
| A | SER150 |
| A | ILE189 |
| A | ARG280 |
| A | PHE283 |
| A | GLY319 |
| A | ASP320 |
| A | MET326 |
| A | LEU327 |
| A | ALA328 |
| A | HIS329 |
| A | TYR359 |
| A | HOH476 |
| A | HOH478 |
| A | HOH489 |
| A | BME500 |
| A | HOH535 |
| A | HOH547 |
| B | HIS452 |
| A | ILE12 |
| A | GLY13 |
| A | GLY15 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NHE A 475 |
| Chain | Residue |
| A | ASN225 |
| A | GLY402 |
| A | HOH536 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE BME B 500 |
| Chain | Residue |
| B | SER168 |
| B | CYS277 |
| B | ARG280 |
| B | FAD480 |
| site_id | AC5 |
| Number of Residues | 37 |
| Details | BINDING SITE FOR RESIDUE FAD B 480 |
| Chain | Residue |
| A | HIS452 |
| A | PRO453 |
| B | ILE12 |
| B | GLY13 |
| B | GLY15 |
| B | PRO16 |
| B | GLY17 |
| B | ILE35 |
| B | GLU36 |
| B | LYS37 |
| B | ASN38 |
| B | GLY43 |
| B | THR44 |
| B | CYS45 |
| B | VAL48 |
| B | GLY49 |
| B | CYS50 |
| B | LYS54 |
| B | TYR118 |
| B | GLY119 |
| B | ALA147 |
| B | THR148 |
| B | GLY149 |
| B | SER150 |
| B | ARG280 |
| B | PHE283 |
| B | LEU287 |
| B | GLY319 |
| B | ASP320 |
| B | MET326 |
| B | LEU327 |
| B | ALA328 |
| B | HIS329 |
| B | ALA331 |
| B | TYR359 |
| B | BME500 |
| B | HOH505 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NHE B 475 |
| Chain | Residue |
| B | ASN225 |
| B | GLY402 |
| B | MET403 |
| B | PHE474 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE BME C 500 |
| Chain | Residue |
| C | ILE189 |
| C | CYS277 |
| C | GLY279 |
| C | HOH503 |
| C | HOH527 |
| E | HIS160 |
| site_id | AC8 |
| Number of Residues | 39 |
| Details | BINDING SITE FOR RESIDUE FAD C 480 |
| Chain | Residue |
| C | ILE12 |
| C | GLY13 |
| C | GLY15 |
| C | PRO16 |
| C | GLY17 |
| C | GLU36 |
| C | LYS37 |
| C | ASN38 |
| C | GLY43 |
| C | THR44 |
| C | CYS45 |
| C | VAL48 |
| C | GLY49 |
| C | CYS50 |
| C | LYS54 |
| C | GLY117 |
| C | TYR118 |
| C | GLY119 |
| C | ALA147 |
| C | THR148 |
| C | GLY149 |
| C | SER150 |
| C | SER168 |
| C | ILE189 |
| C | ARG280 |
| C | PHE283 |
| C | GLY319 |
| C | ASP320 |
| C | MET326 |
| C | LEU327 |
| C | ALA328 |
| C | HIS329 |
| C | ALA331 |
| C | TYR359 |
| C | HOH476 |
| C | HOH491 |
| C | HOH496 |
| C | HOH504 |
| D | HIS452 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NHE C 475 |
| Chain | Residue |
| C | ASN225 |
| C | GLY402 |
| C | PHE474 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE BME D 500 |
| Chain | Residue |
| D | SER168 |
| D | ILE189 |
| D | CYS277 |
| D | ARG280 |
| D | FAD480 |
| site_id | BC2 |
| Number of Residues | 38 |
| Details | BINDING SITE FOR RESIDUE FAD D 480 |
| Chain | Residue |
| C | HIS452 |
| D | ILE12 |
| D | GLY13 |
| D | GLY15 |
| D | PRO16 |
| D | GLY17 |
| D | GLU36 |
| D | LYS37 |
| D | ASN38 |
| D | GLY43 |
| D | THR44 |
| D | CYS45 |
| D | VAL48 |
| D | GLY49 |
| D | CYS50 |
| D | LYS54 |
| D | TYR118 |
| D | GLY119 |
| D | ALA147 |
| D | THR148 |
| D | GLY149 |
| D | SER150 |
| D | SER168 |
| D | ILE189 |
| D | ARG280 |
| D | PHE283 |
| D | GLY319 |
| D | ASP320 |
| D | MET326 |
| D | LEU327 |
| D | ALA328 |
| D | HIS329 |
| D | TYR359 |
| D | HOH476 |
| D | HOH486 |
| D | HOH489 |
| D | HOH490 |
| D | BME500 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NHE D 475 |
| Chain | Residue |
| D | ASN225 |
| D | PRO387 |
| D | GLY402 |
| D | HOH548 |
Functional Information from PROSITE/UniProt
| site_id | PS00076 |
| Number of Residues | 11 |
| Details | PYRIDINE_REDOX_1 Pyridine nucleotide-disulphide oxidoreductases class-I active site. GGtCLnvGCIP |
| Chain | Residue | Details |
| A | GLY42-PRO52 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P53395 |
| Chain | Residue | Details |
| E | LYS135 | |
| F | LYS135 | |
| C | THR454 | |
| D | THR454 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P53395 |
| Chain | Residue | Details |
| E | LYS141 | |
| A | ALA328 | |
| B | ASN38 | |
| B | LEU56 | |
| B | ILE121 | |
| B | SER150 | |
| B | GLY187 | |
| B | LEU210 | |
| B | GLY245 | |
| B | ARG281 | |
| B | VAL322 | |
| F | LYS141 | |
| B | ALA328 | |
| C | ASN38 | |
| C | LEU56 | |
| C | ILE121 | |
| C | SER150 | |
| C | GLY187 | |
| C | LEU210 | |
| C | GLY245 | |
| C | ARG281 | |
| C | VAL322 | |
| A | ILE121 | |
| C | ALA328 | |
| D | ASN38 | |
| D | LEU56 | |
| D | ILE121 | |
| D | SER150 | |
| D | GLY187 | |
| D | LEU210 | |
| D | GLY245 | |
| D | ARG281 | |
| D | VAL322 | |
| A | SER150 | |
| D | ALA328 | |
| A | GLY187 | |
| A | LEU210 | |
| A | GLY245 | |
| A | ARG281 | |
| A | VAL322 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | SITE: Important for interaction with PDHX and activity of multienzyme pyruvate dehydrogenase complex => ECO:0000269|PubMed:20385101 |
| Chain | Residue | Details |
| A | VAL415 | |
| A | ALA440 | |
| B | VAL415 | |
| B | ALA440 | |
| C | VAL415 | |
| C | ALA440 | |
| D | VAL415 | |
| D | ALA440 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 28 |
| Details | MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:O08749 |
| Chain | Residue | Details |
| A | VAL33 | |
| B | MET89 | |
| B | LEU99 | |
| B | ASN110 | |
| B | GLU377 | |
| B | ILE472 | |
| C | VAL33 | |
| C | PHE71 | |
| C | MET89 | |
| C | LEU99 | |
| C | ASN110 | |
| A | PHE71 | |
| C | GLU377 | |
| C | ILE472 | |
| D | VAL33 | |
| D | PHE71 | |
| D | MET89 | |
| D | LEU99 | |
| D | ASN110 | |
| D | GLU377 | |
| D | ILE472 | |
| A | MET89 | |
| A | LEU99 | |
| A | ASN110 | |
| A | GLU377 | |
| A | ILE472 | |
| B | VAL33 | |
| B | PHE71 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 20 |
| Details | MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:O08749 |
| Chain | Residue | Details |
| A | GLN126 | |
| B | ASN397 | |
| C | GLN126 | |
| C | ASP133 | |
| C | ASN240 | |
| C | THR244 | |
| C | ASN397 | |
| D | GLN126 | |
| D | ASP133 | |
| D | ASN240 | |
| D | THR244 | |
| A | ASP133 | |
| D | ASN397 | |
| A | ASN240 | |
| A | THR244 | |
| A | ASN397 | |
| B | GLN126 | |
| B | ASP133 | |
| B | ASN240 | |
| B | THR244 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:O08749 |
| Chain | Residue | Details |
| A | GLY252 | |
| B | GLY252 | |
| C | GLY252 | |
| D | GLY252 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q6P6R2 |
| Chain | Residue | Details |
| A | GLY264 | |
| B | GLY264 | |
| C | GLY264 | |
| D | GLY264 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 8 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:O08749 |
| Chain | Residue | Details |
| A | PRO313 | |
| A | PRO387 | |
| B | PRO313 | |
| B | PRO387 | |
| C | PRO313 | |
| C | PRO387 | |
| D | PRO313 | |
| D | PRO387 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | GLY384 | |
| B | GLY384 | |
| C | GLY384 | |
| D | GLY384 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | GLY469 | |
| B | GLY469 | |
| C | GLY469 | |
| D | GLY469 |
