3RLH

Crystal structure of a class II phospholipase D from Loxosceles intermedia venom

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Dermonecrotic toxin LiSicTox-alphaIA1a	polymer	328	36601.9	1	UniProt (P0CE80) Pfam (PF13653)	Loxosceles intermedia	Dermonecrotic toxin 1,DT1,LiRecDT1,P1,Phospholipase D,PLD,Sphingomyelin phosphodiesterase D 1,SMD 1,SMase D 1,Sphingomyelinase D 1
2	B (A)	(2r,5r)-5-amino-2-hydroxy-5-(hydroxymethyl)-1,3,2lambda~5~-dioxaphosphinan-2-one	non-polymer		183.1	1	Chemie (A1BTZ)
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
5	E, F (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
6	G, H (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7	I (A)	water	water		18.0	190	Chemie (HOH)

Sequence modifications

A: -25 - 284 (UniProt: P0CE80)

PDB	External Database	Details
Met -48	-	initiating methionine
Gly -47	-	expression tag
Ser -46	-	expression tag
Ser -45	-	expression tag
His -44	-	expression tag
His -43	-	expression tag
His -42	-	expression tag
His -41	-	expression tag
His -40	-	expression tag
His -39	-	expression tag
Ser -38	-	expression tag
Ser -37	-	expression tag
Gly -36	-	expression tag
Leu -35	-	expression tag
Val -34	-	expression tag
Pro -33	-	expression tag
Arg -32	-	expression tag
Gly -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
Met -28	-	expression tag
Leu -27	-	expression tag
Glu -26	-	expression tag
Asp 277	Glu 298	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36601.9
Non-Polymers*	Number of molecules	7
	Total formula weight	694.0
All*	Total formula weight	37295.9

*Water molecules are not included.