3RFS

Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Internalin B, repeat modules, Variable lymphocyte receptor B	polymer	272	30425.5	2	UniProt (D2P9A6) UniProt (Q4G1L3) Pfam (PF13855) Pfam (PF11921) In PDB	Listeria monocytogenes (Inshore hagfish)
2	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3		water	water		18.0	218	Chemie (HOH)

Sequence modifications

A, B: 1 - 65 (UniProt: D2P9A6)

PDB	External Database	Details
Ala 24	Asp 59	CONFLICT

A, B: 66 - 179 (PDB: 3RFS)
A, B: 180 - 266 (UniProt: Q4G1L3)

PDB	External Database	Details
His 267	-	EXPRESSION TAG
His 268	-	EXPRESSION TAG
His 269	-	EXPRESSION TAG
His 270	-	EXPRESSION TAG
His 271	-	EXPRESSION TAG
His 272	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60851.0
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	60899.6

*Water molecules are not included.