3RFS
Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.733, 107.693, 71.277 |
Unit cell angles | 90.00, 91.06, 90.00 |
Refinement procedure
Resolution | 33.830 - 1.700 |
R-factor | 0.19911 |
Rwork | 0.197 |
R-free | 0.24206 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.097 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.830 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 49492 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 0.1M Tris-HCl, 30%(w/v) polyethylene glycol 4000, 0.2M magnesium chloride hexahydrate, pH 8.5, VAPOR DIFFUSION, temperature 293K |