3QTC
Crystal structure of the catalytic domain of MmOmeRS, an O-methyl tyrosyl-tRNA synthetase evolved from Methanosarcina mazei PylRS, complexed with O-methyl tyrosine and AMP-PNP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Pyrrolysyl-tRNA synthetase | polymer | 290 | 33495.4 | 1 | UniProt (Q8PWY1) Pfam (PF01409) In PDB | Methanosarcina mazei (Methanosarcina frisia) | Pyrrolysine-tRNA ligase, PylRS |
2 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
3 | A | O-methyl-L-tyrosine | non-polymer | 195.2 | 1 | Chemie (0A1) | |||
4 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
5 | A | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
6 | water | water | 18.0 | 235 | Chemie (HOH) |
Sequence modifications
A: 185 - 454 (UniProt: Q8PWY1)
PDB | External Database | Details |
---|---|---|
Met 165 | - | expression tag |
Lys 166 | - | expression tag |
His 167 | - | expression tag |
His 168 | - | expression tag |
His 169 | - | expression tag |
His 170 | - | expression tag |
His 171 | - | expression tag |
His 172 | - | expression tag |
His 173 | - | expression tag |
His 174 | - | expression tag |
Gly 175 | - | expression tag |
Gly 176 | - | expression tag |
Leu 177 | - | expression tag |
Val 178 | - | expression tag |
Pro 179 | - | expression tag |
Arg 180 | - | expression tag |
Gly 181 | - | expression tag |
Ser 182 | - | expression tag |
His 183 | - | expression tag |
Gly 184 | - | expression tag |
Thr 302 | Ala 302 | engineered mutation |
Val 346 | Asn 346 | engineered mutation |
Trp 348 | Cys 348 | engineered mutation |
Phe 384 | Tyr 384 | engineered mutation |
Leu 401 | Val 401 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 33495.4 | |
Non-Polymers* | Number of molecules | 9 |
Total formula weight | 1060.4 | |
All* | Total formula weight | 34555.8 |