3QQC

Crystal structure of archaeal Spt4/5 bound to the RNAP clamp domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA-directed RNA polymerase subunit b, DNA-directed RNA polymerase subunit a', DNA-directed RNA polymerase subunit A''	polymer	436	49409.9	1	UniProt (Q8U0M3) UniProt (Q8U0M4) UniProt (Q8U0M5) Pfam (PF04560) Pfam (PF04997)	Pyrococcus furiosus
2	B (D)	Transcription antitermination protein nusG	polymer	163	18101.0	1	UniProt (Q8TZK1) Pfam (PF03439) Pfam (PF00467)	Pyrococcus furiosus
3	C (E)	DNA-directed RNA polymerase, subunit e''	polymer	61	6965.0	1	UniProt (Q8U440) Pfam (PF06093)	Pyrococcus furiosus
4	D, E, F, G (A, E)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A: 2 - 64 (UniProt: Q8U0M3)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 65	-	linker
Gly 66	-	linker

A: 67 - 390 (UniProt: Q8U0M4)

PDB	External Database	Details
Gly 391	-	linker
Ala 392	-	linker
Gly 393	-	linker
Ser 394	-	linker
Gly 395	-	linker
Ala 396	-	linker
Gly 397	-	linker
Ser 398	-	linker
Gly 399	-	linker

A: 400 - 437 (UniProt: Q8U0M5)

PDB	External Database	Details
Lys 438	-	expression tag
His 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
His 442	-	expression tag
His 443	-	expression tag
His 444	-	expression tag

D: 1 - 152 (UniProt: Q8TZK1)

PDB	External Database	Details
Ala 153	-	expression tag
Ala 154	-	expression tag
Ala 155	-	expression tag
Leu 156	-	expression tag
Glu 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag
His 163	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	74475.9
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	74737.5

*Water molecules are not included.