3QOL
Crystal structure of Staphylococcal nuclease variant D+PHS/V23E at pH 6 determined at 100 K
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Thermonuclease | polymer | 143 | 16173.4 | 1 | UniProt (P00644) Pfam (PF00565) UniProt (by SIFTS) (Q8NXI6) | Staphylococcus aureus | TNase, Micrococcal nuclease, Staphylococcal nuclease, Nuclease B, Nuclease A |
| 2 | B (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 3 | C (A) | THYMIDINE-3',5'-DIPHOSPHATE | non-polymer | 402.2 | 1 | Chemie (THP) | |||
| 4 | D (A) | water | water | 18.0 | 76 | Chemie (HOH) |
Sequence modifications
A: 1 - 149 (UniProt: P00644)
| PDB | External Database | Details |
|---|---|---|
| Glu 23 | Val 63 | engineered mutation |
| - | Thr 84 | deletion |
| - | Lys 85 | deletion |
| - | His 86 | deletion |
| - | Pro 87 | deletion |
| - | Lys 88 | deletion |
| - | Lys 89 | deletion |
| Phe 50 | Gly 90 | engineered mutation |
| Asn 51 | Val 91 | engineered mutation |
| Gly 117 | Pro 157 | engineered mutation |
| Leu 124 | His 164 | engineered mutation |
| Ala 128 | Ser 168 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 16173.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 442.3 | |
| All* | Total formula weight | 16615.7 |
