3QOL

Crystal structure of Staphylococcal nuclease variant D+PHS/V23E at pH 6 determined at 100 K

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 150

Chain	Residue
A	ASP21
A	ASP40
A	THR41
A	GLU43
A	THP151
A	HOH157
A	HOH167

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site_id	AC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE THP A 151

Chain	Residue
A	LYS84
A	TYR85
A	ARG87
A	LEU89
A	TYR113
A	TYR115
A	LYS127
A	CA150
A	HOH156
A	HOH157
A	HOH159
A	HOH162
A	HOH173
A	HOH190
A	HOH195
A	HOH220
A	ARG35
A	ASP40

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Functional Information from PROSITE/UniProt

site_id	PS01284
Number of Residues	11
Details	TNASE_2 Thermonuclease family signature 2. DKYGRgLAyIY

Chain	Residue	Details
A	ASP83-TYR93

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:288045

Chain	Residue	Details
A	ASP83
A	TYR91
A	GLU135

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site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING:

Chain	Residue	Details
A	ALA69
A	GLY88
A	LEU89

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site_id	SWS_FT_FI3
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000250

Chain	Residue	Details
A	ARG35
A	GLU43
A	TYR93

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site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00272

Chain	Residue	Details
A	ASP21
A	ASP40
A	THR41

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 165

Chain	Residue	Details
A	ALA69	metal ligand
A	ASP83	electrostatic stabiliser, hydrogen bond donor
A	GLY88	metal ligand
A	LEU89	metal ligand
A	TYR91	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	GLU135	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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