3QML

The structural analysis of Sil1-Bip complex reveals the mechanism for Sil1 to function as a novel nucleotide exchange factor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	78 kDa glucose-regulated protein homolog	polymer	390	42453.1	2	UniProt (P16474) Pfam (PF00012)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	GRP-78, Immunoglobulin heavy chain-binding protein homolog, BiP
2	C, D (C, D)	Nucleotide exchange factor SIL1	polymer	315	36700.3	2	UniProt (Q08199) Pfam (PF16782)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Protein SLS1
3	E, F, I, J, K... (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	8	Chemie (PO4)
4	G, H, M (A, C)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	P, Q, R, S (A, B, C, D)	water	water		18.0	206	Chemie (HOH)

Sequence modifications

A, B: 43 - 426 (UniProt: P16474)

PDB	External Database	Details
Gly 37	-	expression tag
Met 38	-	expression tag
Ser 39	-	expression tag
His 40	-	expression tag
Ala 41	-	expression tag
Ser 42	-	expression tag

C, D: 113 - 421 (UniProt: Q08199)

PDB	External Database	Details
Gly 107	-	expression tag
Met 108	-	expression tag
Ser 109	-	expression tag
His 110	-	expression tag
Ala 111	-	expression tag
Ser 112	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	158306.8
Non-Polymers*	Number of molecules	11
	Total formula weight	832.7
All*	Total formula weight	159139.4

*Water molecules are not included.