3QML
The structural analysis of Sil1-Bip complex reveals the mechanism for Sil1 to function as a novel nucleotide exchange factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-03 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 226.353, 116.586, 55.844 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.129 - 2.310 |
| R-factor | 0.2133 |
| Rwork | 0.210 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.098 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_351)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.710 | |
| Number of reflections | 60106 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 91.7 | 79.1 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 298 | 100 mM HEPES buffer, 25% (w/v) PEG4K, 0.2 M Ammonium sulfate, pH 7.0, EVAPORATION, temperature 298K |
