3QK1

Crystal Structure of Enterokinase-like Trypsin Variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cationic trypsin	polymer	229	24310.5	1	UniProt (P00760) Pfam (PF00089) In PDB	Bos taurus (bovine)	Beta-trypsin, Alpha-trypsin chain 1, Alpha-trypsin chain 2
2	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
3	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
5	A	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
6	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7	A	BENZAMIDINE	non-polymer		120.2	1	Chemie (BEN)
8		water	water		18.0	126	Chemie (HOH)

Sequence modifications

A: 16 - 245 (UniProt: P00760)

PDB	External Database	Details
Lys 96	Ser 101	engineered mutation
Arg 97	Asn 102	engineered mutation
Arg 98	Thr 103	engineered mutation
Lys 99	Leu 104	engineered mutation
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	24310.5
Non-Polymers*	Number of molecules	11
	Total formula weight	857.9
All*	Total formula weight	25168.3

*Water molecules are not included.