3QEO

S74E-R104M-D133A dCK variant in complex with L-deoxythymidine and UDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Deoxycytidine kinase	polymer	279	32544.5	2	UniProt (P27707) Pfam (PF01712)	Homo sapiens (human)	dCK
2	C, E (A, B)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP) PubChem (1158) PubChem (6031) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (53486216) PubChem (60098857) PubChem (57448939) PubChem (3084486) PubChem (91746160) PubChem (6101632) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) PubChem (6604175) PubChem (11811538) PubChem (12049241)
3	D, F (A, B)	L-deoxythymidine	non-polymer		242.2	2	Chemie (LLT)
4	G, H (A, B)	water	water		18.0	156	Chemie (HOH)

Sequence modifications

A, B: 1 - 260 (UniProt: P27707)

PDB	External Database	Details
Met -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 9	Cys 9	engineered mutation
Ser 45	Cys 45	engineered mutation
Ser 59	Cys 59	engineered mutation
Glu 74	Ser 74	engineered mutation
Met 104	Arg 104	engineered mutation
Ala 133	Asp 133	engineered mutation
Ser 146	Cys 146	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65089.1
Non-Polymers*	Number of molecules	4
	Total formula weight	1292.8
All*	Total formula weight	66381.9

*Water molecules are not included.