3QE4

An evolved aminoacyl-tRNA Synthetase with atypical polysubstrate specificity

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Tyrosyl-tRNA synthetase	polymer	312	35763.8	2	UniProt (Q57834) Pfam (PF00579)	Methanocaldococcus jannaschii (Methanococcus jannaschii)	Tyrosine--tRNA ligase, TyrRS
2	C, D (A, B)	4-cyano-L-phenylalanine	non-polymer		190.2	2	Chemie (4CF)
3	E, F (A, B)	water	water		18.0	173	Chemie (HOH)

Sequence modifications

A, B: 1 - 306 (UniProt: Q57834)

PDB	External Database	Details
Leu 32	Tyr 32	engineered mutation
Val 65	Leu 65	engineered mutation
Trp 108	Phe 108	engineered mutation
Met 109	Gln 109	engineered mutation
Gly 158	Asp 158	engineered mutation
Ala 159	Ile 159	engineered mutation
Leu 307	-	expression tag
Ile 308	-	expression tag
Arg 309	-	expression tag
Lys 310	-	expression tag
Arg 311	-	expression tag
Leu 312	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	71527.6
Non-Polymers*	Number of molecules	2
	Total formula weight	380.4
All*	Total formula weight	71908.0

*Water molecules are not included.