3QE4
An evolved aminoacyl-tRNA Synthetase with atypical polysubstrate specificity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-04-02 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.524, 68.937, 82.089 |
| Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
| Resolution | 44.450 - 2.300 |
| R-factor | 0.2369 |
| Rwork | 0.233 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j1u |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.065 |
| Number of reflections | 25170 |
| <I/σ(I)> | 16.9 |
| Completeness [%] | 96.2 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | 0.2 M ammonium tartrate, 20% PEG3350, 20 mM Tris-HCl, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
