3QC9

Crystal structure of cross-linked bovine GRK1 T8C/N480C double mutant complexed with ADP and Mg

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Rhodopsin kinase	polymer	543	61421.0	4	UniProt (P28327) Pfam (PF00615) Pfam (PF00069)	Bos taurus (bovine cow domestic cattle domestic cow)	RK, G protein-coupled receptor kinase 1
2	E, I, M, P (A, B, C, D)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
3	F, G, J, K, N... (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
4	H, L (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	S, T, U, V (A, B, C, D)	water	water		18.0	113	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 535 (UniProt: P28327)

PDB	External Database	Details
Cys 8	Thr 8	engineered mutation
Cys 480	Asn 480	engineered mutation
Val 536	-	expression tag
Asp 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	245684.0
Non-Polymers*	Number of molecules	14
	Total formula weight	2087.4
All*	Total formula weight	247771.5

*Water molecules are not included.