3QC9

Crystal structure of cross-linked bovine GRK1 T8C/N480C double mutant complexed with ADP and Mg

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Rhodopsin kinase	polymer	543	61421.0	4	UniProt (P28327) Pfam (PF00615) Pfam (PF00069) In PDB	Bos taurus (bovine cow domestic cattle domestic cow)	RK, G protein-coupled receptor kinase 1
2	A, B, C, D	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
3	A, B, C, D	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
4	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5		water	water		18.0	113	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 535 (UniProt: P28327)

PDB	External Database	Details
Cys 8	Thr 8	engineered mutation
Cys 480	Asn 480	engineered mutation
Val 536	-	expression tag
Asp 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	245684.0
Non-Polymers*	Number of molecules	14
	Total formula weight	2087.4
All*	Total formula weight	247771.5

*Water molecules are not included.