3QC9

Crystal structure of cross-linked bovine GRK1 T8C/N480C double mutant complexed with ADP and Mg

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001750	cellular_component	photoreceptor outer segment
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004703	molecular_function	G protein-coupled receptor kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006468	biological_process	protein phosphorylation
A	0007165	biological_process	signal transduction
A	0007601	biological_process	visual perception
A	0009966	biological_process	regulation of signal transduction
A	0016020	cellular_component	membrane
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0022400	biological_process	regulation of opsin-mediated signaling pathway
A	0050254	molecular_function	rhodopsin kinase activity
A	0097381	cellular_component	photoreceptor disc membrane
B	0000166	molecular_function	nucleotide binding
B	0001750	cellular_component	photoreceptor outer segment
B	0004672	molecular_function	protein kinase activity
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0004703	molecular_function	G protein-coupled receptor kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0006468	biological_process	protein phosphorylation
B	0007165	biological_process	signal transduction
B	0007601	biological_process	visual perception
B	0009966	biological_process	regulation of signal transduction
B	0016020	cellular_component	membrane
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0022400	biological_process	regulation of opsin-mediated signaling pathway
B	0050254	molecular_function	rhodopsin kinase activity
B	0097381	cellular_component	photoreceptor disc membrane
C	0000166	molecular_function	nucleotide binding
C	0001750	cellular_component	photoreceptor outer segment
C	0004672	molecular_function	protein kinase activity
C	0004674	molecular_function	protein serine/threonine kinase activity
C	0004703	molecular_function	G protein-coupled receptor kinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0006468	biological_process	protein phosphorylation
C	0007165	biological_process	signal transduction
C	0007601	biological_process	visual perception
C	0009966	biological_process	regulation of signal transduction
C	0016020	cellular_component	membrane
C	0016301	molecular_function	kinase activity
C	0016740	molecular_function	transferase activity
C	0022400	biological_process	regulation of opsin-mediated signaling pathway
C	0050254	molecular_function	rhodopsin kinase activity
C	0097381	cellular_component	photoreceptor disc membrane
D	0000166	molecular_function	nucleotide binding
D	0001750	cellular_component	photoreceptor outer segment
D	0004672	molecular_function	protein kinase activity
D	0004674	molecular_function	protein serine/threonine kinase activity
D	0004703	molecular_function	G protein-coupled receptor kinase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0006468	biological_process	protein phosphorylation
D	0007165	biological_process	signal transduction
D	0007601	biological_process	visual perception
D	0009966	biological_process	regulation of signal transduction
D	0016020	cellular_component	membrane
D	0016301	molecular_function	kinase activity
D	0016740	molecular_function	transferase activity
D	0022400	biological_process	regulation of opsin-mediated signaling pathway
D	0050254	molecular_function	rhodopsin kinase activity
D	0097381	cellular_component	photoreceptor disc membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ADP A 700

Chain	Residue
A	LEU193
A	THR265
A	MET267
A	LEU321
A	ASP332
A	HOH545
A	HOH546
A	HOH548
A	HOH550
A	MG800
A	MG801
A	GLY194
A	ARG195
A	GLY196
A	GLY197
A	VAL201
A	ALA214
A	LYS216
A	MET264

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 800

Chain	Residue
A	ASP332
A	HOH545
A	HOH546
A	HOH547
A	ADP700

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 801

Chain	Residue
A	ASP332
A	HOH548
A	HOH549
A	HOH550
A	HOH551
A	ADP700

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 544

Chain	Residue
A	GLY199
A	GLU200
A	ASN219
A	ARG222
A	ASP259

---

site_id	AC5
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ADP B 700

Chain	Residue
B	LEU193
B	GLY194
B	ARG195
B	GLY196
B	GLY197
B	VAL201
B	ALA214
B	LYS216
B	MET264
B	THR265
B	MET267
B	LEU321
B	ASP332
B	HOH545
B	HOH546
B	HOH548
B	HOH550
B	MG800
B	MG801

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 800

Chain	Residue
B	ASP332
B	HOH545
B	HOH546
B	HOH547
B	ADP700

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG B 801

Chain	Residue
B	ASP332
B	GLY334
B	HOH548
B	HOH549
B	HOH550
B	HOH551
B	ADP700

---

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL B 544

Chain	Residue
B	SER244
B	ARG329

---

site_id	AC9
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ADP C 700

Chain	Residue
B	HOH553
C	GLY194
C	ARG195
C	GLY197
C	VAL201
C	ALA214
C	LYS216
C	MET264
C	THR265
C	MET267
C	LEU321
C	ASP332
C	HOH545
C	HOH547
C	HOH549
C	MG800
C	MG801

---

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 800

Chain	Residue
B	HOH553
C	ASP332
C	HOH545
C	HOH546
C	ADP700

---

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG C 801

Chain	Residue
C	ASP332
C	HOH547
C	HOH548
C	HOH549
C	HOH550
C	ADP700

---

site_id	BC3
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ADP D 700

Chain	Residue
D	GLY197
D	VAL201
D	ALA214
D	LYS216
D	MET264
D	THR265
D	MET267
D	LEU321
D	ASP332
D	HOH544
D	HOH545
D	HOH546
D	HOH547
D	HOH549
D	MG800
D	MG801
D	LEU193
D	GLY194
D	ARG195

---

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG D 800

Chain	Residue
D	ASP332
D	HOH544
D	HOH545
D	HOH546
D	ADP700

---

site_id	BC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG D 801

Chain	Residue
D	ASP332
D	HOH547
D	HOH548
D	HOH549
D	HOH550
D	ADP700

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGRGGFGEVFaCqmkatgkl..........YACK

Chain	Residue	Details
A	LEU193-LYS216

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IiYrDLKpeNVLL

Chain	Residue	Details
A	ILE310-LEU322

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	342
Details	Domain: {"description":"RGS","evidences":[{"source":"PROSITE-ProRule","id":"PRU00171","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1060
Details	Domain: {"description":"Protein kinase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"18339619","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	32
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"18339619","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"18339619","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---