3QBV

Structure of designed orthogonal interaction between CDC42 and nucleotide exchange domains of intersectin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Cell division control protein 42 homolog	polymer	178	19784.7	2	UniProt (P60953) Pfam (PF00071) In PDB	Homo sapiens (human)	G25K GTP-binding protein
2	B, D	Intersectin-1	polymer	351	40736.3	2	UniProt (Q15811) Pfam (PF00621) Pfam (PF16652) In PDB	Homo sapiens (human)	SH3 domain-containing protein 1A, SH3P17
3	A, C	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
4		water	water		18.0	115	Chemie (HOH)

Sequence modifications

A, C: 1 - 178 (UniProt: P60953)

PDB	External Database	Details
Arg 56	Phe 56	engineered mutation
Lys 163	Arg 163	variant

B, D: 1229 - 1579 (UniProt: Q15811)

PDB	External Database	Details
Glu 1373	Ser 1373	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	121042.1
Non-Polymers*	Number of molecules	2
	Total formula weight	886.4
All*	Total formula weight	121928.5

*Water molecules are not included.