3PV8

Crystal Structure of Bacillus DNA Polymerase I Large Fragment Bound to DNA and ddTTP-dA in Closed Conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	DNA polymerase I	polymer	592	67490.3	2	UniProt (Q5KWC1) Pfam (PF22619) Pfam (PF00476)	Geobacillus kaustophilus
2	C, E (B, E)	DNA (5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(2DT))-3')	polymer	9	2650.8	2
3	D, F (C, F)	DNA (5'-D(*C*AP*TP*AP*AP*GP*AP*GP*TP*CP*AP*GP*G)-3')	polymer	13	4024.6	2
4	G, J (A, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	H, K (A, D)	2',3'-DIDEOXY-THYMIDINE-5'-TRIPHOSPHATE	non-polymer		466.2	2	Chemie (D3T)
6	I, L, M (A, D)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
7	N, O, P, Q, R... (A, D, B, C, E...)	water	water		18.0	1823	Chemie (HOH)

Sequence modifications

A, D: 285 - 876 (UniProt: Q5KWC1)

PDB	External Database	Details
Ala 598	Asp 600	engineered mutation
Tyr 710	Phe 712	engineered mutation
His 823	Arg 825	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	148331.4
Non-Polymers*	Number of molecules	7
	Total formula weight	1269.1
All*	Total formula weight	149600.5

*Water molecules are not included.