3PUU

Crystal Structure of Glu121Gln mutant of E. coli Aminopeptidase N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Aminopeptidase N	polymer	891	101440.2	1	UniProt (P04825) Pfam (PF17900) Pfam (PF01433) Pfam (PF11940) Pfam (PF17432) In PDB	Escherichia coli	Alpha-aminoacylpeptide hydrolase
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	A	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
5		water	water		18.0	752	Chemie (HOH)

Sequence modifications

A: 1 - 870 (UniProt: P04825)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Gln 121	Glu 121	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	101440.2
Non-Polymers*	Number of molecules	13
	Total formula weight	1202.3
All*	Total formula weight	102642.5

*Water molecules are not included.