3PUU

Crystal Structure of Glu121Gln mutant of E. coli Aminopeptidase N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	120.764, 120.764, 170.844
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.330 - 2.150
R-factor	0.15372
Rwork	0.152
R-free	0.18717
Starting model (for MR)	2hpo
RMSD bond length	0.016
RMSD bond angle	1.427
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.2.0019)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.082
Number of reflections	151714
<I/σ(I)>	25.5
Completeness [%]	100.0	100
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	298	1.0M ammonium sulfate, 50mM Hepes, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K