3PO5
Structure of a mutant of the large fragment of DNA polymerase I from Thermus Auqaticus in complex with an abasic site and ddATP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | DNA polymerase I | polymer | 540 | 60951.0 | 1 | UniProt (P19821) Pfam (PF09281) Pfam (PF00476) In PDB | Thermus aquaticus | Taq polymerase 1 |
| 2 | B | DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(2DA))-3') | polymer | 12 | 3641.4 | 1 | |||
| 3 | C | DNA (5'-D(P*(3DR)P*TP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3') | polymer | 13 | 3850.5 | 1 | |||
| 4 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 2 | Chemie (PGE) | |||
| 6 | A | 2',3'-dideoxyadenosine triphosphate | non-polymer | 475.2 | 1 | Chemie (DDS) | |||
| 7 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 8 | water | water | 18.0 | 131 | Chemie (HOH) |
Sequence modifications
A: 293 - 832 (UniProt: P19821)
| PDB | External Database | Details |
|---|---|---|
| Lys 614 | Ile 614 | engineered mutation |
| Lys 747 | Met 747 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 68442.8 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 891.9 | |
| All* | Total formula weight | 69334.8 |
