3PO4
Structure of a mutant of the large fragment of DNA polymerase I from Thermus aquaticus in complex with a blunt-ended DNA and ddATP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase I | polymer | 540 | 60951.0 | 1 | UniProt (P19821) Pfam (PF09281) Pfam (PF00476) | Thermus aquaticus | Taq polymerase 1 |
| 2 | B (B) | DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(2DA))-3') | polymer | 12 | 3641.4 | 1 | |||
| 3 | C (C) | DNA (5'-D(*TP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3') | polymer | 12 | 3670.4 | 1 | |||
| 4 | D, H, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 5 | E (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 6 | F (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | G (A) | 2',3'-dideoxyadenosine triphosphate | non-polymer | 475.2 | 1 | Chemie (DDS) | |||
| 8 | J, K, L (A, B, C) | water | water | 18.0 | 548 | Chemie (HOH) |
Sequence modifications
A: 293 - 832 (UniProt: P19821)
| PDB | External Database | Details |
|---|---|---|
| Lys 614 | Ile 614 | engineered mutation |
| Lys 747 | Met 747 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 68262.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 663.2 | |
| All* | Total formula weight | 68925.9 |
