3PNC
Ternary crystal structure of a polymerase lambda variant with a GT mispair at the primer terminus and sodium at catalytic metal site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase lambda | polymer | 329 | 36736.0 | 1 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (human) | Pol Lambda, DNA polymerase beta-2, Pol beta2, DNA polymerase kappa |
| 2 | B (B) | 5'-D(*CP*AP*GP*TP*AP*G)-3' | polymer | 6 | 1833.2 | 1 | |||
| 3 | C (C) | 5'-D(*CP*GP*GP*CP*CP*TP*TP*AP*CP*TP*G)-3' | polymer | 11 | 3325.2 | 1 | |||
| 4 | D (D) | 5'-D(*GP*CP*CP*G)-3' | polymer | 4 | 1191.8 | 1 | |||
| 5 | E (A) | 2'-deoxy-5'-O-[(R)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]guanosine | non-polymer | 505.2 | 1 | Chemie (1GC) | |||
| 6 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | G, H, J (A, B) | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 8 | I (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 9 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 310 | Chemie (HOH) |
Sequence modifications
A: 242 - 575 (UniProt: Q9UGP5)
| PDB | External Database | Details |
|---|---|---|
| Lys 463 | Ser 463 | SEE REMARK 999 |
| Gly 464 | Gln 464 | SEE REMARK 999 |
| - | Glu 465 | SEE REMARK 999 |
| - | Glu 466 | SEE REMARK 999 |
| - | Asn 467 | SEE REMARK 999 |
| - | Gly 468 | SEE REMARK 999 |
| - | Gln 469 | SEE REMARK 999 |
| Glu 470 | Gln 470 | SEE REMARK 999 |
| Thr 471 | Gln 471 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 43086.3 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 720.6 | |
| All* | Total formula weight | 43806.9 |
