3PML

crystal structure of a polymerase lambda variant with a dGTP analog opposite a templating T

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA polymerase lambda	polymer	329	36736.0	2	UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791)	Homo sapiens (human)	Pol Lambda, DNA polymerase beta-2, Pol beta2, DNA polymerase kappa
2	C, E (C, E)	5'-D(*CP*GP*GP*CP*TP*GP*TP*AP*CP*TP*G)-3'	polymer	11	3365.2	2
3	D, G (D, G)	5'-D(P*GP*CP*CP*G)-3'	polymer	4	1191.8	2
4	F, H (F, H)	5'-D(*CP*AP*GP*TP*AP*C)-3'	polymer	6	1793.2	2
5	I, M (A, B)	2'-deoxy-5'-O-[(R)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]guanosine	non-polymer		505.2	2	Chemie (1GC)
6	J, K, N, O (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	L, P, Q (A, B)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
8	R, S, T, U, V... (A, B, C, E, F...)	water	water		18.0	168	Chemie (HOH)

Sequence modifications

A, B: 242 - 575 (UniProt: Q9UGP5)

PDB	External Database	Details
Lys 463	Ser 463	SEE REMARK 999
Gly 464	Gln 464	SEE REMARK 999
-	Glu 465	SEE REMARK 999
-	Glu 466	SEE REMARK 999
-	Asn 467	SEE REMARK 999
-	Gly 468	SEE REMARK 999
-	Gln 469	SEE REMARK 999
Glu 470	Gln 470	SEE REMARK 999
Thr 471	Gln 471	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	86172.5
Non-Polymers*	Number of molecules	9
	Total formula weight	1176.6
All*	Total formula weight	87349.1

*Water molecules are not included.