3PLA
Crystal structure of a catalytically active substrate-bound box C/D RNP from Sulfolobus solfataricus
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, K (A, B, K) | Pre mRNA splicing protein | polymer | 388 | 44168.5 | 3 | UniProt (Q97ZH3) Pfam (PF21572) Pfam (PF01798) | Sulfolobus solfataricus | NOP5 |
| 2 | C, D, L (C, D, L) | 50S ribosomal protein L7Ae | polymer | 130 | 14075.3 | 3 | UniProt (D0KRE2) | Sulfolobus solfataricus | |
| 3 | E, F, M (E, F, M) | Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase | polymer | 232 | 26439.4 | 3 | UniProt (P58032) Pfam (PF01269) | Sulfolobus solfataricus | FIB |
| 4 | G, H, N (G, H, N) | C/D guide RNA | polymer | 40 | 12852.7 | 3 | synthetic construct | ||
| 5 | I, J, O (I, J, O) | RNA (5'-R(*CP*CP*AP*UP*GP*AP*GP*UP*GP*U)-3') | polymer | 10 | 3177.9 | 3 | synthetic construct | ||
| 6 | P, Q, R (E, F, M) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 3 | Chemie (SAH) PubChem (16760024) PubChem (193) PubChem (13792) PubChem (177678424) PubChem (189149) PubChem (439155) PubChem (25210495) PubChem (21125603) PubChem (44361184) PubChem (25203782) PubChem (46936206) PubChem (50989525) PubChem (51380914) PubChem (52224398) PubChem (57544532) PubChem (52224400) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (5284331) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (71170554) PubChem (13231890) PubChem (6419189) PubChem (118374891) PubChem (118560068) PubChem (126969526) PubChem (129105892) PubChem (164157219) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) |
Sequence modifications
A, B, K: 1 - 380 (UniProt: Q97ZH3)
C, D, L: 1 - 130 (UniProt: D0KRE2)
E, F, M: 1 - 232 (UniProt: P58032)
| PDB | External Database | Details |
|---|---|---|
| Val 2 | Met 2 | engineered mutation |
| His 381 | - | expression tag |
| His 382 | - | expression tag |
| His 383 | - | expression tag |
| His 384 | - | expression tag |
| His 385 | - | expression tag |
| His 386 | - | expression tag |
| His 387 | - | expression tag |
| His 388 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp 2 | Asn 2 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 2 | Ser 2 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 302141.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1153.2 | |
| All* | Total formula weight | 303294.6 |
