3PLA

Crystal structure of a catalytically active substrate-bound box C/D RNP from Sulfolobus solfataricus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, K (A, B, K)	Pre mRNA splicing protein	polymer	388	44168.5	3	UniProt (Q97ZH3) Pfam (PF21572) Pfam (PF01798)	Sulfolobus solfataricus	NOP5
2	C, D, L (C, D, L)	50S ribosomal protein L7Ae	polymer	130	14075.3	3	UniProt (D0KRE2) Pfam (PF01248)	Sulfolobus solfataricus
3	E, F, M (E, F, M)	Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase	polymer	232	26439.4	3	UniProt (P58032) Pfam (PF01269)	Sulfolobus solfataricus	FIB
4	G, H, N (G, H, N)	C/D guide RNA	polymer	40	12852.7	3		synthetic construct
5	I, J, O (I, J, O)	RNA (5'-R(*CP*CP*AP*UP*GP*AP*GP*UP*GP*U)-3')	polymer	10	3177.9	3		synthetic construct
6	P, Q, R (E, F, M)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	3	Chemie (SAH)

Sequence modifications

A, B, K: 1 - 380 (UniProt: Q97ZH3)

PDB	External Database	Details
Val 2	Met 2	engineered mutation
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag

C, D, L: 1 - 130 (UniProt: D0KRE2)

PDB	External Database	Details
Asp 2	Asn 2	engineered mutation

E, F, M: 1 - 232 (UniProt: P58032)

PDB	External Database	Details
Ala 2	Ser 2	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	302141.4
Non-Polymers*	Number of molecules	3
	Total formula weight	1153.2
All*	Total formula weight	303294.6

*Water molecules are not included.