3PLA
Crystal structure of a catalytically active substrate-bound box C/D RNP from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-15 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9789 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 241.199, 241.199, 145.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.910 - 3.150 |
R-factor | 0.24928 |
Rwork | 0.248 |
R-free | 0.27761 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3ICX 3id6 3id5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.324 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.200 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.095 | 0.536 |
Number of reflections | 70682 | |
<I/σ(I)> | 16.3 | 2.3 |
Completeness [%] | 95.1 | 94.4 |
Redundancy | 4.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 2.0M (NH4)2SO4, 2% PEG 400, 10mM MgCl2, 0.1M HEPES-Na (pH 6.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K |