3PIE

Crystal structure of the 5'->3' exoribonuclease Xrn1, E178Q mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	5'->3' EXORIBONUCLEASE (Xrn1)	polymer	1155	132344.8	4	UniProt (Q6CJ09) Pfam (PF03159) Pfam (PF17846) Pfam (PF18332) Pfam (PF18334) Pfam (PF18129) In PDB	Kluyveromyces lactis (Yeast)

Sequence modifications

A, B, C, D: 1 - 463 (UniProt: Q6CJ09)

PDB	External Database	Details
Gln 178	Glu 178	engineered mutation
Leu 1246	-	expression tag
Glu 1247	-	expression tag
His 1248	-	expression tag
His 1249	-	expression tag
His 1250	-	expression tag
His 1251	-	expression tag
His 1252	-	expression tag
His 1253	-	expression tag

A, B, C, D: 503 - 984 (UniProt: Q6CJ09)

PDB	External Database	Details
Unk 469	-	SEE REMARK 999
Unk 470	-	SEE REMARK 999
Unk 471	-	SEE REMARK 999
Unk 472	-	SEE REMARK 999
Unk 473	-	SEE REMARK 999
Unk 474	-	SEE REMARK 999
Unk 475	-	SEE REMARK 999
Unk 476	-	SEE REMARK 999
Unk 477	-	SEE REMARK 999
Unk 478	-	SEE REMARK 999
Unk 479	-	SEE REMARK 999
Unk 480	-	SEE REMARK 999
Unk 481	-	SEE REMARK 999
Unk 482	-	SEE REMARK 999
Unk 483	-	SEE REMARK 999
Unk 484	-	SEE REMARK 999
Unk 485	-	SEE REMARK 999
Unk 486	-	SEE REMARK 999
Unk 487	-	SEE REMARK 999

A, B, C, D: 1082 - 1245 (UniProt: Q6CJ09)

PDB	External Database	Details
Unk 1036	-	SEE REMARK 999
Unk 1037	-	SEE REMARK 999
Unk 1038	-	SEE REMARK 999
Unk 1039	-	SEE REMARK 999
Unk 1040	-	SEE REMARK 999
Unk 1041	-	SEE REMARK 999
Unk 1042	-	SEE REMARK 999
Unk 1043	-	SEE REMARK 999
Unk 1044	-	SEE REMARK 999
Unk 1045	-	SEE REMARK 999
Unk 1046	-	SEE REMARK 999
Unk 1047	-	SEE REMARK 999
Unk 1048	-	SEE REMARK 999
Unk 1049	-	SEE REMARK 999
Unk 1050	-	SEE REMARK 999
Unk 1051	-	SEE REMARK 999
Unk 1052	-	SEE REMARK 999
Unk 1053	-	SEE REMARK 999
Unk 1054	-	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	529379.2
All*	Total formula weight	529379.2

*Water molecules are not included.