3PIE
Crystal structure of the 5'->3' exoribonuclease Xrn1, E178Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 1 |
Unit cell lengths | 115.720, 132.289, 143.890 |
Unit cell angles | 110.07, 105.70, 103.75 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.24441 |
Rwork | 0.241 |
R-free | 0.30476 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.679 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 156952 | |
Completeness [%] | 97.2 | 96.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris, 0.9M LiCl, 13.5% PEG 6000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |