3PHA
The crystal structure of the W169Y mutant of alpha-glucosidase (gh31 family) from Ruminococcus obeum atcc 29174 in complex with acarbose
Replaces: 3NXMEntity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | alpha-glucosidase | polymer | 666 | 77426.4 | 4 | UniProt (A5ZY13) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365) In PDB | Blautia obeum ATCC 29174 | |
| 2 | E, F, G, H | 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 645.6 | 4 | In PDB BIRD (PRD_900007) GlyTouCan (G66431MI) | alpha-acarbose | ||
| 3 | water | water | 18.0 | 601 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 663 (UniProt: A5ZY13)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Tyr 169 | Trp 169 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 309705.7 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 2582.4 | |
| All* | Total formula weight | 312288.1 |
