3P28

Structure of a Circular Permutant of Green Fluorescent Protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Green fluorescent protein	polymer	241	27239.6	1	UniProt (P42212) In PDB	Aequorea victoria (Jellyfish)
2		water	water		18.0	262	Chemie (HOH)

Sequence modifications

A: 2 - 181 (UniProt: P42212)

PDB	External Database	Details
Met 1	-	expression tag
Gys 18	Ser 65	chromophore
Gys 18	Tyr 66	chromophore
Gys 18	Gly 67	chromophore
Arg 32	Gln 80	engineered mutation
Ser 51	Phe 99	engineered mutation
Thr 105	Met 153	engineered mutation
Ala 115	Val 163	engineered mutation
Lys 158	Ala 206	engineered mutation

A: 195 - 241 (UniProt: P42212)

PDB	External Database	Details
Gly 182	-	linker
Thr 183	-	linker
Gly 184	-	linker
Ser 185	-	linker
Arg 186	-	linker
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag
His 190	-	expression tag
His 191	-	expression tag
His 192	-	expression tag
Ser 193	-	expression tag
Arg 194	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27239.6
All*	Total formula weight	27239.6

*Water molecules are not included.