3P28

Structure of a Circular Permutant of Green Fluorescent Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-06-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	41.456, 71.028, 76.894
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.490 - 1.800
R-factor	0.18926
Rwork	0.187
R-free	0.23725
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.888
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASES
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.490	1.860
High resolution limit [Å]	1.800	1.800
Rmerge		0.364
Number of reflections	20615
<I/σ(I)>		2.1
Completeness [%]	99.2	100
Redundancy		2.67

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	279	0.07 M Na-acetate trihydrate pH 4.6, 5.6% PEG 4000, and 30 % Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 279K