3OSR

Maltose-bound maltose sensor engineered by insertion of circularly permuted green fluorescent protein into E. coli maltose binding protein at position 311

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Maltose-binding periplasmic protein,Green fluorescent protein	polymer	655	72691.8	2	UniProt (P0AEY0) UniProt (P42212) Pfam (PF01547) Pfam (PF01353)	Escherichia coli O157:H7	MBP,MMBP,Maltodextrin-binding protein
2	C, D (C, D)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	E, F (A, B)	water	water		18.0	559	Chemie (HOH)

Sequence modifications

A, B: 1 - 311 (UniProt: P0AEY0)

PDB	External Database	Details
Met -35	-	expression tag
Arg -34	-	expression tag
Gly -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Gly -25	-	expression tag
Met -24	-	expression tag
Ala -23	-	expression tag
Ser -22	-	expression tag
Met -21	-	expression tag
Thr -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Gln -17	-	expression tag
Gln -16	-	expression tag
Met -15	-	expression tag
Gly -14	-	expression tag
Arg -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Tyr -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Arg -3	-	expression tag
Trp -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

A, B: 314 - 405 (UniProt: P42212)

PDB	External Database	Details
Arg 325	Lys 158	conflict
Ala 330	Val 163	conflict
Gly 342	Ser 175	conflict
Tyr 347	Asp 180	conflict
Lys 373	Ala 206	conflict
Leu 398	His 231	conflict

A, B: 414 - 558 (UniProt: P42212)

PDB	External Database	Details
Gly 406	-	linker
Gly 407	-	linker
Thr 408	-	linker
Gly 409	-	linker
Gly 410	-	linker
Ser 411	-	linker
Met 412	-	linker
Val 413	-	linker
Leu 476	Phe 64	conflict
C12 478	Ser 65	chromophore
C12 478	Tyr 66	chromophore
C12 478	Gly 67	chromophore
Ile 505	Val 93	conflict
Phe 557	Tyr 145	conflict
Asn 559	-	linker
Pro 560	-	linker

A, B: 561 - 619 (UniProt: P0AEY0)

PDB	External Database	Details
Gly 312	-	linker
Gly 313	-	linker
Asp 609	Ala 386	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	145383.6
Branched	Number of molecules	2
	Total formula weight	684.6
All*	Total formula weight	146068.2

*Water molecules are not included.