3OSR
Maltose-bound maltose sensor engineered by insertion of circularly permuted green fluorescent protein into E. coli maltose binding protein at position 311
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.825, 74.813, 171.504 |
| Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
| Resolution | 34.240 - 2.000 |
| R-factor | 0.18488 |
| Rwork | 0.183 |
| R-free | 0.22573 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 3EK4 1anf |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.868 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.240 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.118 | |
| Number of reflections | 96467 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 296 | 0.1 M Sodium acetate pH 4.6, 8% w/v polyethylene glycol 4000, VAPOR DIFFUSION, temperature 296K |
