3ORV

Crystal Structure of the Y294H-MauG/pre-Methylamine Dehydrogenase Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Methylamine utilization protein mauG	polymer	373	41121.6	2	UniProt (Q51658) Pfam (PF03150)	Paracoccus denitrificans
2	C, E (C, E)	Methylamine dehydrogenase light chain	polymer	137	15025.6	2	UniProt (P22619) Pfam (PF02975)	Paracoccus denitrificans
3	D, F (D, F)	Methylamine dehydrogenase heavy chain	polymer	386	42449.3	2	UniProt (A1BB97) Pfam (PF06433)	Paracoccus denitrificans
4	G, J (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	H, I, K, L (A, B)	HEME C	non-polymer		618.5	4	Chemie (HEC)
6	M, O, V (B, D, F)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
7	N (D)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
8	P, Q, U (D, E, F)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
9	R, S (F)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
10	T (F)	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
11	AA, BA, W, X, Y... (E, F, A, B, C...)	water	water		18.0	1301	Chemie (HOH)

Sequence modifications

A, B: 1 - 367 (UniProt: Q51658)

PDB	External Database	Details
His 294	Tyr 314	engineered mutation
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag

C, E: 1 - 131 (UniProt: P22619)

PDB	External Database	Details
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	197192.9
Non-Polymers*	Number of molecules	16
	Total formula weight	3879.7
All*	Total formula weight	201072.6

*Water molecules are not included.