3ONW
Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 328 | 37416.6 | 2 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | C, D (C, D) | Regulator of G-protein signaling 14 | polymer | 36 | 4093.6 | 2 | UniProt (O43566) Pfam (PF02188) | RGS14 | |
| 3 | E, G (A, B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 4 | F, H, I (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 5 | J, K, L, M (A, B, C, D) | water | water | 18.0 | 97 | Chemie (HOH) |
Sequence modifications
A, B: 31 - 354 (UniProt: P63096)
| PDB | External Database | Details |
|---|---|---|
| Ser 27 | - | expression tag |
| Asn 28 | - | expression tag |
| Ala 29 | - | expression tag |
| Gly 30 | - | expression tag |
| Leu 147 | Gln 147 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 83020.5 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1174.6 | |
| All* | Total formula weight | 84195.1 |
