3ONW

Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.

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Functional Information from GO Data

ChainGOidnamespacecontents
A0099738cellular_componentcell cortex region
A0005813cellular_componentcentrosome
A0005737cellular_componentcytoplasm
A0070062cellular_componentextracellular exosome
A0005834cellular_componentheterotrimeric G-protein complex
A0005765cellular_componentlysosomal membrane
A0045121cellular_componentmembrane raft
A0030496cellular_componentmidbody
A0005730cellular_componentnucleolus
A0005634cellular_componentnucleus
A0005886cellular_componentplasma membrane
A0001664molecular_functionG protein-coupled receptor binding
A0031821molecular_functionG protein-coupled serotonin receptor binding
A0031683molecular_functionG-protein beta/gamma-subunit complex binding
A0019003molecular_functionGDP binding
A0005525molecular_functionGTP binding
A0032794molecular_functionGTPase activating protein binding
A0003924molecular_functionGTPase activity
A0000287molecular_functionmagnesium ion binding
A0007193biological_processadenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
A0007188biological_processadenylate cyclase-modulating G protein-coupled receptor signaling pathway
A0007049biological_processcell cycle
A0051301biological_processcell division
A1904322biological_processcellular response to forskolin
A0007186biological_processG protein-coupled receptor signaling pathway
A0050805biological_processnegative regulation of synaptic transmission
A1904778biological_processpositive regulation of protein localization to cell cortex
A0006457biological_processprotein folding
A0043949biological_processregulation of cAMP-mediated signaling
A0060236biological_processregulation of mitotic spindle organization
A0043434biological_processresponse to peptide hormone
B0099738cellular_componentcell cortex region
B0005813cellular_componentcentrosome
B0005737cellular_componentcytoplasm
B0070062cellular_componentextracellular exosome
B0005834cellular_componentheterotrimeric G-protein complex
B0005765cellular_componentlysosomal membrane
B0045121cellular_componentmembrane raft
B0030496cellular_componentmidbody
B0005730cellular_componentnucleolus
B0005634cellular_componentnucleus
B0005886cellular_componentplasma membrane
B0001664molecular_functionG protein-coupled receptor binding
B0031821molecular_functionG protein-coupled serotonin receptor binding
B0031683molecular_functionG-protein beta/gamma-subunit complex binding
B0019003molecular_functionGDP binding
B0005525molecular_functionGTP binding
B0032794molecular_functionGTPase activating protein binding
B0003924molecular_functionGTPase activity
B0000287molecular_functionmagnesium ion binding
B0007193biological_processadenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
B0007188biological_processadenylate cyclase-modulating G protein-coupled receptor signaling pathway
B0007049biological_processcell cycle
B0051301biological_processcell division
B1904322biological_processcellular response to forskolin
B0007186biological_processG protein-coupled receptor signaling pathway
B0050805biological_processnegative regulation of synaptic transmission
B1904778biological_processpositive regulation of protein localization to cell cortex
B0006457biological_processprotein folding
B0043949biological_processregulation of cAMP-mediated signaling
B0060236biological_processregulation of mitotic spindle organization
B0043434biological_processresponse to peptide hormone
C0030054cellular_componentcell junction
C0005813cellular_componentcentrosome
C0005737cellular_componentcytoplasm
C0030425cellular_componentdendrite
C0043197cellular_componentdendritic spine
C0098978cellular_componentglutamatergic synapse
C0005874cellular_componentmicrotubule
C0016604cellular_componentnuclear body
C0005634cellular_componentnucleus
C0005886cellular_componentplasma membrane
C0016605cellular_componentPML body
C0014069cellular_componentpostsynaptic density
C0045211cellular_componentpostsynaptic membrane
C0005819cellular_componentspindle
C0000922cellular_componentspindle pole
C0001965molecular_functionG-protein alpha-subunit binding
C0005092molecular_functionGDP-dissociation inhibitor activity
C0032794molecular_functionGTPase activating protein binding
C0005096molecular_functionGTPase activator activity
C0003924molecular_functionGTPase activity
C0008017molecular_functionmicrotubule binding
C0019901molecular_functionprotein kinase binding
C0030159molecular_functionreceptor signaling complex scaffold activity
C0051301biological_processcell division
C0007059biological_processchromosome segregation
C0007186biological_processG protein-coupled receptor signaling pathway
C0035556biological_processintracellular signal transduction
C0007612biological_processlearning
C0007616biological_processlong-term memory
C0060291biological_processlong-term synaptic potentiation
C0000278biological_processmitotic cell cycle
C0070373biological_processnegative regulation of ERK1 and ERK2 cascade
C0045744biological_processnegative regulation of G protein-coupled receptor signaling pathway
C0043407biological_processnegative regulation of MAP kinase activity
C0031914biological_processnegative regulation of synaptic plasticity
C0006913biological_processnucleocytoplasmic transport
C0048008biological_processplatelet-derived growth factor receptor signaling pathway
C0050769biological_processpositive regulation of neurogenesis
C0043620biological_processregulation of DNA-templated transcription in response to stress
C0008277biological_processregulation of G protein-coupled receptor signaling pathway
C0006979biological_processresponse to oxidative stress
C0007051biological_processspindle organization
C0008542biological_processvisual learning
C0010070biological_processzygote asymmetric cell division
D0030054cellular_componentcell junction
D0005813cellular_componentcentrosome
D0005737cellular_componentcytoplasm
D0030425cellular_componentdendrite
D0043197cellular_componentdendritic spine
D0098978cellular_componentglutamatergic synapse
D0005874cellular_componentmicrotubule
D0016604cellular_componentnuclear body
D0005634cellular_componentnucleus
D0005886cellular_componentplasma membrane
D0016605cellular_componentPML body
D0014069cellular_componentpostsynaptic density
D0045211cellular_componentpostsynaptic membrane
D0005819cellular_componentspindle
D0000922cellular_componentspindle pole
D0001965molecular_functionG-protein alpha-subunit binding
D0005092molecular_functionGDP-dissociation inhibitor activity
D0032794molecular_functionGTPase activating protein binding
D0005096molecular_functionGTPase activator activity
D0003924molecular_functionGTPase activity
D0008017molecular_functionmicrotubule binding
D0019901molecular_functionprotein kinase binding
D0030159molecular_functionreceptor signaling complex scaffold activity
D0051301biological_processcell division
D0007059biological_processchromosome segregation
D0007186biological_processG protein-coupled receptor signaling pathway
D0035556biological_processintracellular signal transduction
D0007612biological_processlearning
D0007616biological_processlong-term memory
D0060291biological_processlong-term synaptic potentiation
D0000278biological_processmitotic cell cycle
D0070373biological_processnegative regulation of ERK1 and ERK2 cascade
D0045744biological_processnegative regulation of G protein-coupled receptor signaling pathway
D0043407biological_processnegative regulation of MAP kinase activity
D0031914biological_processnegative regulation of synaptic plasticity
D0006913biological_processnucleocytoplasmic transport
D0048008biological_processplatelet-derived growth factor receptor signaling pathway
D0050769biological_processpositive regulation of neurogenesis
D0043620biological_processregulation of DNA-templated transcription in response to stress
D0008277biological_processregulation of G protein-coupled receptor signaling pathway
D0006979biological_processresponse to oxidative stress
D0007051biological_processspindle organization
D0008542biological_processvisual learning
D0010070biological_processzygote asymmetric cell division
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Functional Information from PDB Data

site_idNumber of ResiduesDetails
AC119BINDING SITE FOR RESIDUE GDP A 401
ChainResidue
AALA41
AGLU43
ASER44
AGLY45
ALYS46
ASER47
ATHR48
AASP150
ASER151
AARG176
AARG178
AASN269
ALYS270
AASP272
ALEU273
ACYS325
AALA326
ATHR327
CARG516

AC221BINDING SITE FOR RESIDUE GDP B 401
ChainResidue
BHOH23
BALA41
BGLU43
BSER44
BGLY45
BLYS46
BSER47
BTHR48
BASP150
BSER151
BARG176
BARG178
BASN269
BLYS270
BASP272
BLEU273
BCYS325
BALA326
BTHR327
BHOH357
DARG516

AC34BINDING SITE FOR RESIDUE SO4 B 1
ChainResidue
BTYR154
BGLN172
BARG176
BHOH365

AC43BINDING SITE FOR RESIDUE SO4 B 2
ChainResidue
BARG242
DSER510
DGLY511

AC52BINDING SITE FOR RESIDUE SO4 A 3
ChainResidue
ASER293
AASN294

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Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
GDP_3onw_A_40125GUANOSINE-5'-DIPHOSPHATE binding site
ChainResidueligand
AALA41-THR48GDP: GUANOSINE-5'-DIPHOSPHATE
AASN149-SER151GDP: GUANOSINE-5'-DIPHOSPHATE
ALEU175-ARG176GDP: GUANOSINE-5'-DIPHOSPHATE
AARG178GDP: GUANOSINE-5'-DIPHOSPHATE
AASP200GDP: GUANOSINE-5'-DIPHOSPHATE
AASN269-LEU273GDP: GUANOSINE-5'-DIPHOSPHATE
ATHR324-THR327GDP: GUANOSINE-5'-DIPHOSPHATE
CARG516GDP: GUANOSINE-5'-DIPHOSPHATE

GDP_3onw_B_40125GUANOSINE-5'-DIPHOSPHATE binding site
ChainResidueligand
BALA41-THR48GDP: GUANOSINE-5'-DIPHOSPHATE
BASN149-SER151GDP: GUANOSINE-5'-DIPHOSPHATE
BLEU175-ARG176GDP: GUANOSINE-5'-DIPHOSPHATE
BARG178GDP: GUANOSINE-5'-DIPHOSPHATE
BASP200GDP: GUANOSINE-5'-DIPHOSPHATE
BASN269-LEU273GDP: GUANOSINE-5'-DIPHOSPHATE
BTHR324-THR327GDP: GUANOSINE-5'-DIPHOSPHATE
DARG516GDP: GUANOSINE-5'-DIPHOSPHATE

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Functional Information from PROSITE/UniProt

site_idNumber of ResiduesDetails
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Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails
SWS_FT_FI117GTP.
ChainResidueDetails
AGLY14-SER21
AASP174-GLN178
AASN243-ASP246

SWS_FT_FI217GTP.
ChainResidueDetails
BGLY14-SER21
BASP174-GLN178
BASN243-ASP246

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Catalytic Information from CSA

site_idNumber of ResiduesDetails