3OL6

Poliovirus polymerase elongation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (A, E, I, M)	Polymerase	polymer	471	53761.2	4	UniProt (B3VQP5) Pfam (PF00680)	Human poliovirus 1
2	B, F, J, N (B, F, J, N)	RNA (5'-R(*AP*AP*GP*UP*CP*UP*CP*CP*AP*GP*GP*UP*CP*UP*CP*UP*CP*GP*UP*CP*CP*GP*GP*AP*AP*A)-3')	polymer	26	8280.0	4
3	C, G, K, O (C, G, K, O)	RNA (5'-R(*GP*CP*CP*CP*GP*GP*AP*CP*GP*AP*GP*AP*GP*A)-3')	polymer	14	4563.8	4
4	D, H, L, P (D, H, L, P)	RNA (5'-R(*GP*GP*GP*AP*GP*AP*UP*GP*A)-3')	polymer	9	2974.9	4
5	AA, FA, Q, W (I, M, A, E)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
6	BA, CA, DA, EA, GA... (I, M, A, E, G)	ISOPROPYL ALCOHOL	non-polymer		60.1	16	Chemie (IPA)
7	KA, LA, MA, NA, OA... (A, B, C, D, E...)	water	water		18.0	759	Chemie (HOH)

Sequence modifications

A, E, I, M: 1 - 461 (UniProt: B3VQP5)

PDB	External Database	Details
Asp 446	Leu 2194	engineered mutation
Gly 462	-	expression tag
Ser 463	-	expression tag
Ser 464	-	expression tag
Ser 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	278319.4
Non-Polymers*	Number of molecules	20
	Total formula weight	1223.2
All*	Total formula weight	279542.5

*Water molecules are not included.