3O7O

Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Endoglucanase	polymer	264	30678.5	2	UniProt (Q9S5X8) Pfam (PF01670)	Thermotoga maritima
2	C, D, E, F, G... (A, B)	BROMIDE ION	non-polymer		79.9	7	Chemie (BR)
3	J, K (A, B)	water	water		18.0	72	Chemie (HOH)

Sequence modifications

A, B: 1 - 258 (UniProt: Q9S5X8)

PDB	External Database	Details
Cys 188	Asp 188	engineered mutation
His 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61357.1
Non-Polymers*	Number of molecules	7
	Total formula weight	559.3
All*	Total formula weight	61916.4

*Water molecules are not included.