3O0X

Structural basis of carbohydrate recognition by calreticulin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Calreticulin	polymer	273	31300.0	2	UniProt (Q3TVD2) Pfam (PF00262) UniProt (by SIFTS) (P14211)	Mus musculus (mouse)
2	C, D (C, D)	alpha-D-glucopyranose-(1-3)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose	branched		666.6	2	In PDB GlyTouCan (G82436MB)
3	E, F (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	G, H (A, B)	water	water		18.0	374	Chemie (HOH)

Sequence modifications

A, B: 18 - 295 (UniProt: Q3TVD2)

PDB	External Database	Details
Gly 15	-	expression tag
Ser 16	-	expression tag
Mse 17	-	expression tag
Ser 163	Cys 163	engineered mutation

A, B: 301 - 368 (UniProt: Q3TVD2)

PDB	External Database	Details
Gly 296	-	linker
Ser 297	-	linker
Gly 298	-	linker
Ser 299	-	linker
Gly 300	-	linker
Leu 369	-	expression tag
Glu 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	62600.1
Branched	Number of molecules	2
	Total formula weight	1333.2
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	64013.4

*Water molecules are not included.